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Human Synaptopodin ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80297

Novus Biologicals, part of Bio-Techne
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NBP2-80297

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 ul)

Sensitivity

0.094 ng/mL

Assay Range

0.156-10 ng/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human SYNPO in samples. No significant cross-reactivity or interference between Human SYNPO and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.09%

Inter-Assay Precision (Precision between assays) CV% < 4.13%

Recovery for Human Synaptopodin ELISA Kit (Colorimetric)

Recovery

86-110%

Linearity

Human Synaptopodin ELISA Kit (Colorimetric)

Scientific Data Images for Human Synaptopodin ELISA Kit (Colorimetric)

Human Synaptopodin ELISA Kit (Colorimetric)

ELISA: Human Synaptopodin ELISA Kit (Colorimetric) [NBP2-80297] -

ELISA: Human Synaptopodin ELISA Kit (Colorimetric) [NBP2-80297] - Standard Curve Reference

Kit Contents for Human Synaptopodin ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100X)
  • Concentrated HRP Conjugate (100X)
  • Concentrated Wash Buffer (25X)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Synaptopodin

Synaptopodin, a prolin-rich actin-binding protein with 2 binding sites for actin, represents a new class of actin-binding proteins which has first been localized in podocytes and a subset of telencephalic postsynaptic densities. In human tissue synaptopodin has a molecular weight of 73.7 kD and pI of 9.38 (calculated from sequence data); in mouse the corresponding data are 74 kD, pI 9.27. In SDS-PAGE the antigen appears as 100 kD polypeptide in brain and 110 kD polypeptide in kidney (the difference might be attributed to posttranslational modifications).

Alternate Names

SYNPO

Gene Symbol

SYNPO

UniProt

Additional Synaptopodin Products

Product Documents for Human Synaptopodin ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Synaptopodin ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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