Human t-Plasminogen Activator/tPA ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-91260

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

cell culture supernatant, serum (10 uL)

Sensitivity

6 pg/mL

Assay Range

15.6 - 1,000 pg/mL

View all t-Plasminogen Activator/tPA ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

There was no crossreactivity detected, namely not with human PAI-1 (plasminogen activator inhibitor 1, maximum concentration tested 2000 pg/ml).

Description

This kit is an enzyme-linked immunosorbent assay for quantitative detection.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 3.6%

Inter-Assay Precision (Precision between assays) CV% < 6.5%

Recovery for Human t-Plasminogen Activator/tPA ELISA Kit (Colorimetric)

Recovery

The unspiked serum was used as blank in these experiments. The recovery ranged from 77% to 109% with an overall mean recovery of 98%.

Scientific Data Images for Human t-Plasminogen Activator/tPA ELISA Kit (Colorimetric)

ELISA: Tissue Plasminogen Activator ELISA Kit 96Tests [NBP1-91260]

Kit Contents for Human t-Plasminogen Activator/tPA ELISA Kit (Colorimetric)

  • Adhesive Films
  • Aluminium pouch(es) with a Microwell Plate coated with polyclonal antibody to human t-PA
  • Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  • HRP-Conjugate anti-human t-PA polyclonal antibody
  • Human t-PA Standard lyophilized, 2 ng/mL upon reconstitution
  • Sample Diluent
  • Stop Solution (1M Phosphoric acid)
  • Substrate Solution (tetramethyl-benzidine)
  • Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Formulation

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: t-Plasminogen Activator/tPA

Tissue-type plasminogen activator (t-PA) is a serine protease which occurs in blood plasma, serum, other body fluids, tissues and conditioned media of certain cultured cells. It can convert the inactive proenzyme plasminogen to the active protease plasmin. Plasmin can degrade fibrin, the matrix of a blood clot in a process known as fibrinolysis, leading to dissolution of the clot. Furthermore plasminogen activation is implicated in metastatic spread of malignant cells and in tissue remodelling. Elevated levels of t-PA in serum were shown to occur in relation to retinopathy in type 1 diabetes mellitus. The major field of clinical interest is the field of diseases of the heart. t-PA plasma levels have been shown to be altered with the presence of transplant coronary artery disease in cardiac transplant recipients. A correlation between low serum levels of t-PA activity and thrombotic tendency has been described.

Long Name

Tissue Plasminogen Activator

Alternate Names

Alteplase, PLAT, Reteplase, T-PA, tPlasminogen Activator

Gene Symbol

PLAT

Additional t-Plasminogen Activator/tPA Products

Product Documents for Human t-Plasminogen Activator/tPA ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human t-Plasminogen Activator/tPA ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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