Human Talin2 - Ready-To-Use ELISA Kit (Colorimetric)

Name: Human Talin2 - Ready-To-Use ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP3-40171

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-40171

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Product Specifications
Precision
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Tissue homogenates, cell lysates and other biological fluids (100 uL)

Sensitivity

0.132 ng/mL (example only; lot dependent)

Assay Range

0.312 - 20 ng/mL (example only; lot dependent)

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time.
Assay Length: 3 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)

Scientific Data Images for Human Talin2 - Ready-To-Use ELISA Kit (Colorimetric)

ELISA: Human Talin2 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-40171]

ELISA: Human Talin2 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-40171] - Standard Curve Reference

Kit Contents for Human Talin2 - Ready-To-Use ELISA Kit (Colorimetric)

Detection Solution A
Detection Solution B
Instruction manual
Plate sealer for 96 wells
Pre-coated 96T strip plate
Standard
Standard Diluent
Stop Solution
TMB Substrate
Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Talin2

Talin2 (talus; Latin for ankle, the shape of a focal adhesion plaque complex) is a 240 kDa cytoplasmic focal adhesion molecule. It likely forms dimers that link integrins to the actin cytoskeleton. Human Talin2 is 2542 amino acids (aa) in length. It contains one globular N-terminal FERM domain that binds integrin cytodomains (aa 88-406), followed by an Ala-rich region (aa 861-1193) and a C-terminal actin-binding domain (aa 2321-2530). Human Talin2 has three potential variants. Two show alternate start sites at Met19 and Met1153, while a third has an alternate start site at Met1608 accompanied by a deletion of aa 1748-1790. Over aa's 977-1183, human Talin2 shares 99% and 97% aa identity with canine and mouse Talin2, respectively.

Alternate Names

ILWEQ, TLN2

Gene Symbol

TLN2

Additional Talin2 Products

Product Documents for Human Talin2 - Ready-To-Use ELISA Kit (Colorimetric)

Product Datasheets

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COA

Product Specific Notices for Human Talin2 - Ready-To-Use ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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