TBARS Parameter Assay Kit
R&D Systems, part of Bio-Techne | Catalog # KGE013
Key Product Details
Assay Length
2-3 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (150 µL), Cell Lysates (150 µL), Serum (150 µL), EDTA Plasma (150 µL), Heparin Plasma (150 µL), Urine (150 µL)
Sensitivity
0.055 µM
Assay Range
0-16.7 µM
Product Summary for TBARS Parameter Assay Kit
The Parameter TBARS assay is a 2-3 hour chemical analysis designed to measure TBARS in cell culture supernates, cell lysates, serum, plasma, and urine.
Product Specifications
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
Biotin
Reactivity
Multi-Species
Specificity
TBARS in various samples
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Cell Lysates, EDTA Plasma, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (µM) | 2.57 | 6.07 | 9.26 | 2.39 | 5.71 | 8.76 |
| Standard Deviation | 0.025 | 0.078 | 0.109 | 0.125 | 0.242 | 0.322 |
| CV% | 1.0 | 1.3 | 1.2 | 5.2 | 4.2 | 3.7 |
Recovery for TBARS Parameter Assay Kit
The recovery of MDA spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 101 | 94-110 |
| Cell Lysis Buffer 3 (n=1) | 99 | 96-103 |
| EDTA Plasma (n=4) | 91 | 89-106 |
| Heparin Plasma (n=4) | 99 | 94-108 |
| Serum (n=4) | 98 | 87-105 |
| Urine (n=4) | 99 | 91-106 |
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of MDA, acid treated, and serially diluted with deionized water to produce samples with values within the dynamic range of the assay.
Scientific Data Images for TBARS Parameter Assay Kit
Multi-species TBARS/Thiobarbituric Acid Reactive Substances ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: TBARS/Thiobarbituric Acid Reactive Substances
Alternate Names
Thiobarbituric Acid Reactive Substances
Additional TBARS/Thiobarbituric Acid Reactive Substances Products
Product Documents for TBARS Parameter Assay Kit
Product Specific Notices for TBARS Parameter Assay Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including methanol, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov.