Human Thyroglobulin ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18039

Novus Biologicals, part of Bio-Techne
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NBP3-18039
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.47 ng/mL

Assay Range

0.78-50 ng/mL

View all Thyroglobulin ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Precision

Intra-Assay Precision (Precision within an assay) %CV < 5.26

Inter-Assay Precision (Precision between assays) %CV < 5.97

Recovery for Human Thyroglobulin ELISA Kit (Colorimetric)

Recovery

85-100%

Linearity

Human Thyroglobulin ELISA Kit (Colorimetric)

Scientific Data Images for Human Thyroglobulin ELISA Kit (Colorimetric)

Human Thyroglobulin ELISA Kit (Colorimetric)

ELISA: Human Thyroglobulin ELISA Kit (Colorimetric) [NBP3-18039] -

ELISA: Human Thyroglobulin ELISA Kit (Colorimetric) [NBP3-18039] - Standard Curve Reference

Kit Contents for Human Thyroglobulin ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Thyroglobulin

Thyroglobulin is synthesized by the follicular epithelial cells of the thyroid. Thyroglobulin is the glycoprotein precursor to the thyroid hormones. Its synthesis and metabolism have seemingly wasteful features. It has a molecular weight of 660,000, with 2 identical subunits of MW 300,000 and 10% sugars; yet its complete hydrolysis yields only 2 to 4 molecules of the iodothyroxines T4 and T3. Baas et al. (1986) showed that the TG gene encodes an 8.7-kb mRNA, covers at least 300 kb of genomic DNA and contains at least 37 exons separated by introns as large as 64 kb. A striking structural difference between the 5-prime and 3-prime parts of the gene suggested that it is composed of 2 evolutionarily different regions. The first 30 kb of DNA encodes 3 kb of the mRNA yielding an exon:intron ratio of 1:10, whereas the remaining 270 kb encodes 5.7 kb of the mRNA with a ratio of 1.47.

Alternate Names

AITD3, cog, TDH3, Tg, TGN

Gene Symbol

TG

Additional Thyroglobulin Products

Product Documents for Human Thyroglobulin ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Thyroglobulin ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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