Human TNF RI/TNFRSF1A ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83738

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum, plasma (EDTA, citrate, heparin), urine (10 uL)

Sensitivity

0.05 ng/mL

Assay Range

0.08 - 5.0 ng/mL

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

The cross reactivity and interference of circulating factors of the immune system were evaluated by spiking these proteins at physiologically relevant concentrations into a human TNF RI/TNFRSF1A (60 kDa) positive serum (50% calf serum containing 5 ng/ml TNF RI/TNFRSF1A (60 kDa)). There was no cross reactivity or interference detected, notably not with human TNF alpha (< 10 ng/ml) and TNF beta (< 100 ug/ml)

Description

The human sTNF-R (60 kDa) ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human sTNF-R (60 kDa)

Precision

Intra-Assay Precision (Precision within an assay) CV% < 1.9%

Inter-Assay Precision (Precision between assays) CV% < 8.6%

Recovery for Human TNF RI/TNFRSF1A ELISA Kit (Colorimetric)

Recovery

The recovery ranged from 91% to 96% with an overall mean recovery of 93%. Please see protocol for more specific Spike Recovery details.

Scientific Data Images for Human TNF RI/TNFRSF1A ELISA Kit (Colorimetric)

Sandwich ELISA: Human TNF RI/TNFRSF1A ELISA Kit (Colorimetric) [NBP1-83738] - Representative standard curve for human TNF-R (60 kDa) ELISA. Human TNF-R (60 kDa) was diluted in serial 2-fold steps in Assay buffer (1x). Do not use this standard curve to derive test results. A standard curve must be run foreach group of microwell strips assayed.

Kit Contents for Human TNF RI/TNFRSF1A ELISA Kit (Colorimetric)

  • Adhesive Films
  • Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human Adiponectin
  • Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  • Biotin-Conjugate anti-human Adiponectin monoclonal antibody
  • Human Adiponectin Standard lyophilized, 30 ng/ml upon reconstitution
  • Stop Solution (1M Phosphoric acid)
  • Streptavidin-HRP
  • Substrate Solution (tetramethyl-benzidine)
  • Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: TNF RI/TNFRSF1A

TNF-receptors have been demonstrated on a wide variety of human somatic cells including fibroblasts, endothelial cells, adipocytes, liver membranes, granulocytes and several tumor cell lines. Based on gel filtration experiments the receptor appears to be a complex of different proteins with a molecular weight of 350 kDa. In a variety of cell lines two different types of TNF receptors with 75-80 and 55-60 kDa respectively have been identified. The cDNAs encoding the two different TNF receptors have been cloned. The exact mechanism of signal transduction after binding of TNF to the receptor is still unclear. The extracellular fragment of the 60 kDa TNF receptor, with a molecular mass of about 30 kDa was purified, partially sequenced, and the respective cDNA was cloned. This TNF binding protein is liberated from the intact molecule by proteolytic cleavage and comprises most of the extracellular portion of the receptor, including all three N-glycosylation sites. The present assay provides a simple, rapid, and highly sensitive method for the determination of soluble TNF-R (60 kDa) levels in body fluids or cell culture supernatants. This assay will help to clarify the possible diagnostic and prognostic value of circulating sTNF-R (60 kDa) in various neoplastic and inflammatory diseases.

Long Name

Tumor Necrosis Factor Receptor I

Alternate Names

CD120a, TNFRI, TNFRSF1A

Gene Symbol

TNFRSF1A

Additional TNF RI/TNFRSF1A Products

Product Documents for Human TNF RI/TNFRSF1A ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human TNF RI/TNFRSF1A ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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