Total Nitric Oxide and Nitrate/Nitrite Parameter Assay Kit
R&D Systems, part of Bio-Techne | Catalog # KGE001
Key Product Details
Assay Length
< 1 hour
Sample Type & Volume Required Per Well
Cell Culture Supernates (10 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL), Citrate Plasma (25 µL), Urine (10 µL)
Sensitivity
0.78 µmol/L
Assay Range
3.12-200 µmol/L (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine)
Product Summary for Total Nitric Oxide and Nitrate/Nitrite Parameter Assay Kit
The Parameter Total Nitric Oxide kit has two assay options. Endogenous nitrite is measured in the first option. In the second option, nitrate is converted to nitrite using nitrate reductase and total nitrite is measured. To obtain the nitrate concentration, endogenous nitrite is subtracted from the total nitrite value.
Product Specifications
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Reactivity
Multi-Species
Specificity
Measures Total Nitric Oxide, Nitrite and Nitrate levels in various samples
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
Interference observed with other substances.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (µmol/L) | 30.0 | 77.2 | 138.0 | 31.5 | 79.9 | 138.7 |
| Standard Deviation | 0.76 | 1.10 | 2.20 | 1.46 | 2.91 | 4.78 |
| CV% | 2.5 | 1.4 | 1.6 | 4.6 | 3.6 | 3.4 |
Recovery for Total Nitric Oxide and Nitrate/Nitrite Parameter Assay Kit
The recovery of the nitrate standard spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (Nitrite Assay) (n=10) | 107 | 91-115 |
| Plasma (Nitrate Assay) (n=25) | 98 | 87-110 |
| Plasma (Nitrite Assay) (n=20) | 104 | 92-117 |
| Serum (Nitrate Assay) (n=10) | 95 | 89-101 |
| Serum (Nitrite Assay) (n=10) | 109 | 98-118 |
| Urine (Nitrite Assay) (n=10) | 101 | 87-112 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of the nitrate standard were serially diluted with the Reaction Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Total Nitric Oxide and Nitrate/Nitrite Parameter Assay Kit
Multi-species Total Nitric Oxide and Nitrate/Nitrite ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Nitric Oxide
Additional Nitric Oxide Products
Product Documents for Total Nitric Oxide and Nitrate/Nitrite Parameter Assay Kit
Product Specific Notices for Total Nitric Oxide and Nitrate/Nitrite Parameter Assay Kit
For research use only
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