Rat Tryptophan Hydroxylase 1/TPH-1 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-68167

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.19 ng/mL

Assay Range

0.31 - 20 ng/mL

View all Tryptophan Hydroxylase 1/TPH-1 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat TPH1 in samples. No significant cross-reactivity or interference between Rat TPH1 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.91%

Inter-Assay Precision (Precision between assays) CV% < 5.19%

Recovery for Rat Tryptophan Hydroxylase 1/TPH-1 ELISA Kit (Colorimetric)

Recovery

87-110%

Linearity

Rat Tryptophan Hydroxylase 1/TPH-1 ELISA Kit (Colorimetric)

Scientific Data Images for Rat Tryptophan Hydroxylase 1/TPH-1 ELISA Kit (Colorimetric)

Rat Tryptophan Hydroxylase 1/TPH-1 ELISA Kit (Colorimetric)

ELISA: Rat Tryptophan Hydroxylase 1/TPH-1 ELISA Kit (Colorimetric) [NBP2-68167] -

ELISA: Rat Tryptophan Hydroxylase 1/TPH-1 ELISA Kit (Colorimetric) [NBP2-68167] - Standard Curve Reference

Kit Contents for Rat Tryptophan Hydroxylase 1/TPH-1 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Tryptophan Hydroxylase 1/TPH-1

Tryptophan hydroxylase (TPH) is the rate-limiting enzyme in the biosynthesis of serotonin (5-HT) and might be related to the pathogenesis of major depression (MD). Two isoforms are known, TPH1 and TPH2. (1). TPH1 is phosphorylated by cAMP-dependent protein kinase A (2). Recently, it has been shown that expression of the TPH1 gene is increased in lungs and pulmonary endothelial cells from patients with idiopathic pulmonary arterial hypertension. Results indicate that TPH1 and peripheral serotonin play an essential role in the development of hypoxia-induced elevations in pulmonary pressures and hypoxia-induced pulmonary vascular remodeling. In addition, the results suggest that, in mice, serotonin has differential effects on the pulmonary vasculature and right ventricular hypertrophy (3).

Alternate Names

TPH-1, TPRH

Gene Symbol

TPH1

Additional Tryptophan Hydroxylase 1/TPH-1 Products

Product Documents for Rat Tryptophan Hydroxylase 1/TPH-1 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat Tryptophan Hydroxylase 1/TPH-1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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