Human TWEAK/TNFSF12 ELISA Kit (Colorimetric)

Name: Human TWEAK/TNFSF12 ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP3-06808

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06808

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Product Specifications
Precision
Recovery
Linearity
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

4.69 pg/mL

Assay Range

7.81 - 500 pg/mL

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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human TWEAK/TNFSF12 in samples. No significant cross-reactivity or interference between Human TWEAK/TNFSF12 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) 5.07

Inter-Assay Precision (Precision between assays) 5.22

Recovery for Human TWEAK/TNFSF12 ELISA Kit (Colorimetric)

Recovery

87 - 110 %

Linearity

Scientific Data Images for Human TWEAK/TNFSF12 ELISA Kit (Colorimetric)

ELISA: Human TWEAK/TNFSF12 ELISA Kit (Colorimetric) [NBP3-06808] -

ELISA: Human TWEAK/TNFSF12 ELISA Kit (Colorimetric) [NBP3-06808] - Standard Curve Reference

Kit Contents for Human TWEAK/TNFSF12 ELISA Kit (Colorimetric)

Biotinylated Detection Ab Diluent
Concentrated Biotinylated Detection Ab (100x)
Concentrated HRP Conjugate (100x)
Concentrated Wash Buffer (25x)
HRP Conjugate Diluent
Micro ELISA Plate (Dismountable)
Plate Sealer
Product Manual
Reference Standard
Sample Diluent
Stop Solution
Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: TWEAK/TNFSF12

CD266 is a 14 kD transmembrane protein known as TWEAK receptor (TWEAK-R) or fibroblast growth factor inducible 14 (Fn14). It is member 12A of the tumor necrosis factor receptor superfamily (TNFRSF12A) expressed at low levels in a variety of tissues, such as heart, placenta, lung, muscle, and pancreas. TWEAK-R binds TWEAK (CD255) and interacts with the cytoplasmic proteins TRAF1, 2, and 3. The engagement of Fn14 can induce cell death and may play a role in inflammation, hepatocyte growth, and liver neoplasia. ITEM-4 antibody reacts with both mouse and human Fn14. It is useful for flow cytometric analysis.

Long Name

TNF-like and Weak Inducer of Apoptosis

Alternate Names

TNFSF12

Gene Symbol