Human UBPY/USP8 ELISA Kit (Colorimetric)

Name: Human UBPY/USP8 ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP3-38798

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-38798

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Product Specifications
Precision
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Tissue homogenates, cell lysates and other biological fluids (100 uL)

Sensitivity

0.101 ng/mL (example only; lot dependent)

Assay Range

0.312 - 20 ng/mL (example only; lot dependent)

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

Assay Length: 4.5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)

Scientific Data Images for Human UBPY/USP8 ELISA Kit (Colorimetric)

ELISA: Human UBPY/USP8 ELISA Kit (Colorimetric) [NBP3-38798]

ELISA: Human UBPY/USP8 ELISA Kit (Colorimetric) [NBP3-38798] - Standard Curve Reference

Kit Contents for Human UBPY/USP8 ELISA Kit (Colorimetric)

Detection Reagent A
Detection Reagent B
Diluent Buffer
Instruction manual
Plate sealer for 96 wells
Pre-coated 96T strip plate
Standard
Stop Solution
TMB Substrate
Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: USP8

Ubiquitin Specific Peptidase 8 (USP8), also known as Ubiquitin Isopeptidase Y (UBPY), is a widely expressed deubiquitinating enzyme belonging to the peptidase C19 family. It has a predicted molecular weight of 127.5 kDa. Human USP8 is 1118 amino acids (aa) in length and shares 84% aa sequence identity with the mouse and rat orthologs. It contains an N-terminal MIT domain (aa 33-116) that mediates endosomal localization, CHMP-binding, and maintenance of ESCRT-0. USP8 also has a rhodanese domain (aa 181-319) that binds NRDP1 Ubiquitin ligase (E3), a SH3 domain binding sequence (aa 405-413), and a C-terminal catalytic domain (aa 734-1110). USP8 is a growth-regulated enzyme that controls the internalization and endocytic trafficking of cell surface receptors. Some receptors are targeted for internalization and degradation by ubiquitination. USP8 has been shown to disrupt the down-regulation of multiple receptors, including EGF R/ErbB1, ErbB2/Her2, and Smoothened, via their deubiquitination. Conversely, USP8 appears to have the opposite effect on the trafficking of CXCR4, PAR2, and the delta-opioid receptor. Depletion or catalytic inactivation of USP8 stabilized their expression. It is thought that deubiquitination of these receptors down-stream of the sorting endosome commits them to lysosomal degradation. USP8 can be phosphorylated at Ser680 allowing for 14-3-3-epsilon binding, which subsequently inhibits USP8 activity. Additionally, USP8 undergoes tyrosine phosphorylation at its N-terminus following EGF activation of the EGF R/ErbB1 / ErbB2/Her2 receptor complex.

Long Name

Ubiquitin Specific Protease 8

Alternate Names

UBPY

Gene Symbol

USP8

Additional USP8 Products

Product Documents for Human UBPY/USP8 ELISA Kit (Colorimetric)

Product Datasheets

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COA

Product Specific Notices for Human UBPY/USP8 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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