Human Urocortin ELISA Kit (Colorimetric)

Name: Human Urocortin ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP3-38816

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-38816

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Product Specifications
Precision
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids (100 uL)

Sensitivity

4.9 pg/mL (example only; lot dependent)

Assay Range

15.6 - 1000 pg/mL (example only; lot dependent)

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

Assay Length: 4.5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)

Scientific Data Images for Human Urocortin ELISA Kit (Colorimetric)

ELISA: Human Urocortin ELISA Kit (Colorimetric) [NBP3-38816]

ELISA: Human Urocortin ELISA Kit (Colorimetric) [NBP3-38816] - Standard Curve Reference

Kit Contents for Human Urocortin ELISA Kit (Colorimetric)

Detection Reagent A
Detection Reagent B
Diluent Buffer
Instruction manual
Plate sealer for 96 wells
Pre-coated 96T strip plate
Standard
Stop Solution
TMB Substrate
Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Urocortin

Urocortins 1, 2 and 3 are the mammalian homologs of fish urotensin I. Mammalian urocortin is a member of the corticotropin-releasing hormone(CRH) family that is expressed in a region of the rat midbrain. The distinct expression pattern in discrete brain regions suggests that it influences such behavior as feeding, anxiety and auditory processing. Synthetic human urocortin binds with high affinity to CRH receptors and acts in vitro to release ACTH from dispersed rat anterior pituitary cells. It is suggested that urocortin may be an endogenous CRF-like factor in the brain responsible for the effects of stress on appetite. The gene which encodes urocortin maps to human chromosome 2. Specifically, urocortin III is a specific ligand for CRF type 2 receptor which mediates stress-coping responses. Human urocortin I and III are expressed in the human heart. Urocortin III is also expressed in the pituitary gland, adrenal gland, GI tract, ovary, spleen, brain and kidney.

Alternate Names

UCN, UI, UROC

Gene Symbol

UCN

Additional Urocortin Products

Product Documents for Human Urocortin ELISA Kit (Colorimetric)

Product Datasheets

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COA

Product Specific Notices for Human Urocortin ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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