Human VAP-1/AOC3 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-91261

Novus Biologicals, part of Bio-Techne
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NBP1-91261
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Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum, plasma (EDTA, heparin), buffered solution, amniotic fluid (10 uL (1:100 prediluted))

Sensitivity

19 pg/mL

Assay Range

31.3 - 2,000 pg/mL

View all VAP-1/AOC3 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit detects the soluble form of VAP-1. HAMA may interfere with assays utilizing murine monoclonal antibodies leading to both false positive and false negative results. Serum samples containing antibodies to murine immunoglobulins can still be analysed in such assays when murine immunoglobulins (serum, ascitic fluid, or monoclonal antibodies of irrelevant specificity) are added to the sample.

Description

The human sVAP-1 ELISA is an enzyme-linked immunosorbent assay for quantitative detection.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 3.00%

Inter-Assay Precision (Precision between assays) CV% < 4.50%

Recovery for Human VAP-1/AOC3 ELISA Kit (Colorimetric)

Recovery

The amount of endogenous human VAP-1 in unspiked serum was substracted from the spike values. The recovery ranged from 82% to 117% with an overall mean recovery of 96%.

Scientific Data Images for Human VAP-1/AOC3 ELISA Kit (Colorimetric)

Human VAP-1/AOC3 ELISA Kit [NBP1-91261] - Data for Human sVAP-1 Platinum ELISA

Kit Contents for Human VAP-1/AOC3 ELISA Kit (Colorimetric)

  • Adhesive Films
  • Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human sVAP-1
  • Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  • Biotin-Conjugate anti-human sVAP-1 monoclonal antibody
  • Human sVAP-1 Standard lyophilized, 4 ng/ml upon reconstitution
  • Stop Solution (1M Phosphoric acid)
  • Streptavidin-HRP
  • Substrate Solution (tetramethyl-benzidine)
  • Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: VAP-1/AOC3

Lymphocyte binding to vascular endothelium is a prerequisite for the move- ment of immune cells from the blood into lymphoid tissues and into sites of inflammation. Under inflammatory conditions, cell surface expression of VAP-1 (vascular adhesion protein-1) which is an endothelial sialoglycoprotein, is induced. VAP-1 is a type II transmembrane protein of 84.6 kDa with a single transmembrane domain and N and O-glycosylation sites in the extracellular domain. In vivo, VAP-1 exists predominantly as a 180 kDa homodimer and functions both as an enzyme (monoamine oxidase) and an adhesion molecule for lymphocytes. A circulating form of human Vascular Adhesion Protein-1 (sVAP-1) has been characterized. This sVAP-1 has been shown to be elevated in sera of patients with certain liver diseases and a correlation with the diagnosis of the patients was demonstrated.

Long Name

Vascular Adhesion Protein-1

Alternate Names

AOC3, HPAO, SSAO, VAP1

Gene Symbol

AOC3

Additional VAP-1/AOC3 Products

Product Documents for Human VAP-1/AOC3 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human VAP-1/AOC3 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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