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Rat VEGFR2/KDR/Flk-1 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-78888

Novus Biologicals, part of Bio-Techne
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NBP2-78888

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, and other biological fluids (100 ul)

Sensitivity

0.10 ng/mL

Assay Range

0.16-10 ng/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat VEGFR-2/KDR in samples. No Significant cross-reactivity or interference between Rat VEGFR-2/KDR and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.39%

Inter-Assay Precision (Precision between assays) CV% < 4.78%

Recovery for Rat VEGFR2/KDR/Flk-1 ELISA Kit (Colorimetric)

Recovery

86-108%

Linearity

Rat VEGFR2/KDR/Flk-1 ELISA Kit (Colorimetric)

Scientific Data Images for Rat VEGFR2/KDR/Flk-1 ELISA Kit (Colorimetric)

Rat VEGFR2/KDR/Flk-1 ELISA Kit (Colorimetric)

ELISA: Rat VEGFR2/KDR/Flk-1 ELISA Kit (Colorimetric) [NBP2-78888] -

ELISA: Rat VEGFR2/KDR/Flk-1 ELISA Kit (Colorimetric) [NBP2-78888] - Standard Curve Reference

Kit Contents for Rat VEGFR2/KDR/Flk-1 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: VEGFR2/KDR/Flk-1

Vascular endothelial growth factor receptor 2 (VEGFR2) is a member of a receptor tyrosine kinase family whose activation plays an essential role in a large number of biological processes such as embryonic development, wound healing, cell proliferation, migration and differentiation. Like other common growth factor receptors, VEGF receptor 2 dimerises upon ligand binding and is autophosphorylated at multiple tyrosine residues. These sites can be involved in the regulation of kinase activity or can serve as binding sites for SH2 and phosphotyrosine binding containing signaling proteins. Phosphorylation of Tyrosines 1054 and 1059 in the activation loop is required for activation of VEGF receptor 2 and its intrinsic tyrosine kinase activity.

Defects in the VEGFR2 gene are associated with susceptibility to benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium called hemangioma capillary infantile. HCI is the most common tumor found in infants, found in up to 10% of all births.

Long Name

Vascular Endothelial Growth Factor Receptor 2

Alternate Names

CD309, Flk-1, Flk1, KDR, KRD1, Ly73, VEGF R2

Gene Symbol

KDR

UniProt

Additional VEGFR2/KDR/Flk-1 Products

Product Documents for Rat VEGFR2/KDR/Flk-1 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat VEGFR2/KDR/Flk-1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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