Human VEGFR3/Flt-4 ELISA Kit (Colorimetric)

Name: Human VEGFR3/Flt-4 ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP1-84822

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84822

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Product Specifications
Precision
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum, plasma (citrate), buffered solution (50 uL)

Sensitivity

0.03 ng/mL

Assay Range

0.6 - 40.0 ng/mL

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

The assay detects both natural and recombinant human VEGF-R3/ FLT-4. Cross reactivity and interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human VEGF-R3/FLT-4 positive sample. There was no cross reactivity detected, notably not with VEGF-R1 and VEGF-R2/KDR. Interference was detected for VEGF-C at concentrations > 7 ng/ml, and not for VEGF-D.

Description

The human VEGF-R3/FLT-4 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human VEGF-R3/FLT-4

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.7%

Inter-Assay Precision (Precision between assays) CV% < 5.0%

Scientific Data Images for Human VEGFR3/Flt-4 ELISA Kit (Colorimetric)

ELISA: VEGF Receptor 3 ELISA Kit 96Tests [NBP1-84822]

Kit Contents for Human VEGFR3/Flt-4 ELISA Kit (Colorimetric)

Adhesive Films
Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human VEGF-R3/FLT-4
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Biotin-Conjugate anti-human VEGF-R3/FLT-4 monoclonal antibody
Human VEGF-R3/FLT-4 Standard lyophilized, 80 ng/ml upon reconstitution
Sample Diluent
Stop Solution (1M Phosphoric acid)
Streptavidin-HRP
Substrate Solution (tetramethyl-benzidine)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: VEGFR3/Flt-4

FLT4, a VEGF Receptor type protein kinase, is an endothelial cell-specific receptor. Binding of the extracellular domain of FLT4 to the vascular endothelial growth factor-related protein VEGF-C stimulates tyrosine phosphorylation and mitogenesis of endothelial cells. FLT4 (-/-) mice have been shown to have defective blood vessel development in early embryos and to experience cardiovascular failure at embryonic day 9.5. A mutation in the FLT4 gene has been implicated in human hereditary lymphedema type I. Similarly, a mutation in the conserved catalytic domain of FLT4, in close proximity to the FLT4 mutations in human primary lymphedema, has been linked to the Chy mouse mutant.

Long Name

Vascular Endothelial Growth Factor Receptor 3

Alternate Names

Flt-4, FLT4, VEGF R3

Gene Symbol

FLT4

Additional VEGFR3/Flt-4 Products

Product Documents for Human VEGFR3/Flt-4 ELISA Kit (Colorimetric)

Product Datasheets

Download Product Datasheets

Protocols & Manuals

Download Manual - Human VEGFR3/Flt-4 ELISA Kit (Colorimetric)

COA

SDS

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Product Specific Notices for Human VEGFR3/Flt-4 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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