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Human Vitronectin ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80358

Novus Biologicals, part of Bio-Techne
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NBP2-80358

Key Product Details

Sample Type & Volume Required Per Well

Serum (100 ul)

Sensitivity

15.19 pg/ml

Assay Range

31.25-2000 pg/ml (example only; lot dependent)

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Conjugate

HRP

Reactivity

Human

Specificity

Recognizes both recombinant and natural Human Vitronectin.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 6.2

Inter-Assay Precision (Precision between assays) CV% < 2.4 %

Recovery for Human Vitronectin ELISA Kit (Colorimetric)

Recovery

96 -110%

Scientific Data Images for Human Vitronectin ELISA Kit (Colorimetric)

ELISA: Human Vitronectin ELISA Kit (Colorimetric) [NBP2-80358]

ELISA: Human Vitronectin ELISA Kit (Colorimetric) [NBP2-80358]

ELISA: Human Vitronectin ELISA Kit (Colorimetric) [NBP2-80358] - Example standard curve for Human Vitronectin ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Kit Contents for Human Vitronectin ELISA Kit (Colorimetric)

  • 1. 96 well microplate coated with Capture Antibody
  • 2. Detection Antibody conjugated to HRP
  • 3. Standards
  • 4. Wash Buffer Concentrate
  • 5. Dilution Buffer Concentrate
  • 6. Color Reagent A
  • 7. Color Reagent B
  • 8. Stop Solution

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage is content dependent.

Background: Vitronectin

Vitronectin also called serum spreading factor or complement S-protein, is a member of the pexin family (1). Found in serum and in tissues, Vitronectin exist in two forms; as a single chain (75kDa) and as a clipped form composed of two chains (65kDa and 10kDa). Vitronectin promotes cell adhesion, spreading and migration through specific interactions with cellular integrins, and inhibits cytolysis by the complement C5b-9 complex (2-4). Vitronectin also interacts with several critical proteins that regulate thrombosis and fibrinolysis. Vitronectin is phosphorylated by PKA and CKII (5).

Alternate Names

Complement S-protein, Serum Spreading Factor, Somatomedin B, VTN

Gene Symbol

VTN

Additional Vitronectin Products

Product Documents for Human Vitronectin ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Vitronectin ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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