ZAG Antibody Pair [Biotin]

Sandwich ELISA

0.0156 - 1 ng/ml

Cell Culture Supernatant, Plasma, Serum (100 ul)

100 pg/ml

Human

Sandwich ELISA for the quantitative determination of Human ZAG

100 ul

Biotin

1. Dilute the desired amount of Coating Antibody (COAT) (1mg/ml) (mouse monoclonal antibody) to 2ug/ml in PBS without carrier protein and use it fresh.
2. Dilute the desired amount of Detection Antibody (DET) (0.1mg/ml) (biotinylated mouse monoclonal antibody) to 0.1ug/ml in ELISA Buffer and use it fresh.
3. Reconstitute the Streptavidin-HRP (STREP) with 100ul ELISA Buffer. After reconstitution, prepare aliquots and store the reconstituted STREP at -20C. Avoid freeze/thaw cycles. Dilute the reconstituted Streptavidin-HRP to the working concentration by adding 5ul in 10ml of ELISA Buffer (1:2000).
4. Reconstitute the Standard Protein (STD) (recombinant human ZAG) with 100ul ELISA Buffer to obtain a concentration of 10ug/ml. After reconstitution, prepare aliquots and store the reconstituted standard at -20C. Avoid freeze/thaw cycles. Suggested standard points are 1ng/ml, 0.5ng/ml, 0.25 ng/ml, 0.125 ng/ml, 0.062 ng/ml, 0.031 ng/ml, 0.0156ng/ml and 0 ng/ml.

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.