Human IL-5 ELISpot Development Module

ELISpot development modules are an alternative to ELISpot kits. A basic understanding of ELISpot assay development is required for the successful use of these reagents. Each investigator should optimize the coating conditions, the assay sensitivity, the type of enzyme and substrate, as well as the concentrations of the capture and detection antibodies to achieve desired results. The analyte-specific ELISpot Development Module and the ELISpot Blue Color Module contain the necessary components for analyte detection and visualization, respectively. These modules can be used together but are sold separately. Each module contains enough reagents for at least five 96-well microplates.

Product Features for Human IL-5 ELISpot Development Module

• An economical alternative to ELISpot Kits
• Optimized capture and detection antibody pairings and recommended concentrations save lengthy development time
• Generic development protocols provide direction to start an optimization protocol
• Customize the assay to your specific needs

Module Contents

• Human IL-5 Capture Antibody
• Human IL-5 Biotinylated-conjugated Detection Antibody

Other Reagents Required

• ELISpot Blue Color Module or equivalent (R&D Systems, Catalog # SEL002)
• PBS - 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na₂HPO₄, 1.5 mM KH₂PO₄, pH 7.2 - 7.4, 0.2 µm filtered
• Wash Buffer - 0.05% Tween^® 20 in PBS
• Blocking Buffer - 1% BSA, 5% Sucrose in PBS
• Reagent Diluent - 1% BSA in PBS, pH 7.2 - 7.4, 0.2 µm filtered
• 2 °C – 8 °C refrigerator
• 37 °C CO₂ incubator
• Positive Control - Use Recombinant Human IL-5 or cells known to secrete Human IL-5
• 96-well plates - Nitrocellulose-bottom plates, PVDF-bottom Immunospot^® plates, or flat-bottom polystyrene Immulon^® ELISA plates
• Multi-channel pipette, squirt bottle, manifold dispenser, or automated microplate washer
• Dissection microscope or an automated ELISpot Reader
• Deionized H₂O

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