BI dihydrochloride

Name: BI dihydrochloride
Brand: Tocris Bioscience
SKU: 7466

Tocris Bioscience, part of Bio-Techne | Catalog # 7466

Citations (1)

COA / SDS

DFHBI derivative for imaging of RNA in living cells that bind Broccoli aptamers

Key Product Details

Description:	DFHBI derivative for imaging of RNA in living cells that bind Broccoli aptamers
Chemical Name:	(5Z)-3-(1H-Benzimidazol-7-ylmethyl)-5-[(3,5-difluoro-4-hydroxyphenyl)methylene]-3,5-dihydro-2-methyl-4H-imidazol-4-one dihydrochloride
Purity:	≥98% (HPLC)
Molecular Weight:	441.26

View Full Technical Details for BI dihydrochloride

Technical Data
Citations

Biological Activity for BI dihydrochloride

BI is a DFHBI derivative for imaging of RNA in cells. BI increases brightness and photostability of Broccoli aptamers in cells. BI enables imaging of single Broccoli-tagged mRNAs in living cells. BI exhibits peak excitation of 470 nm and peak fluorescence emission of 505 nm when bound to Broccoli. BI exhibits higher affinity for Broccoli aptamers and a 10.5-fold increase in cellular fluorescence compared with DFHBI-1T (Cat. No. 5610) (K_D values are 51 and 305 nM, respectively).

Licensing Information

Sold under license from Lucerna

Optical Data for BI dihydrochloride

Emission Color	Green
λ_abs	470 nm
λ_em	505 nm
Extinction Coefficient (ε)	33600 M^-1cm^-1
Quantum Yield (φ)	0.669
Cell Permeable	Yes
Application	DFHBI Derivative for Imaging of RNA in Living Cells

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Technical Data for BI dihydrochloride

M. Wt	441.26
Formula	C₁₉H₁₄F₂N₄O₂.2HCl
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	2758438-31-0
InChi Key	YKXWKZLDMGAVJO-ZYMKCKPMSA-N
Smiles	CC1=N/C(C(N1CC2=C3NC=NC3=CC=C2)=O)=C\C4=CC(F)=C(C(F)=C4)O.Cl.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for BI dihydrochloride

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	water	44.13	100
	DMSO	22.06	50

Preparing Stock Solutions for BI dihydrochloride

The following data is based on the product molecular weight 441.26.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.27 mL	11.33 mL	22.66 mL
5 mM	0.45 mL	2.27 mL	4.53 mL
10 mM	0.23 mL	1.13 mL	2.27 mL
50 mM	0.05 mL	0.23 mL	0.45 mL

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References for BI dihydrochloride

References are publications that support the biological activity of the product.

Li Fluorophore promoted RNA folding and photostability enables imaging of single Broccoli-tagged mRNAs in live mammalian cells. Angew.Chem.Int.Ed.Engl. 2020 PMID: 31850609