HBC 530

Name: HBC 530
Brand: Tocris Bioscience
SKU: 7277

Tocris Bioscience, part of Bio-Techne | Catalog # 7277

COA / SDS

GFP fluorophore mimic for imaging RNA in live cells; activated by binding to Pepper aptamers; suitable for confocal and two-photon microscopy

Key Product Details

Description:	GFP fluorophore mimic for imaging RNA in live cells; activated by binding to Pepper aptamers; suitable for confocal and two-photon microscopy
Chemical Name:	4-Cyano-α-[[4-[(2-hydroxyethyl)methylamino]phenyl]methylene]benzeneacetonitrile
Purity:	≥98% (HPLC)
Molecular Weight:	303.37

View Full Technical Details for HBC 530

Technical Data
Citations

Biological Activity for HBC 530

HBC 530 is a mimic of green fluorescent protein (GFP) fluorophore for imaging RNA in live cells. Fluoresces when bound to Pepper aptamer; displays high affinity binding (K_d = 3.5 nM). Suitable for confocal and two-photon microscopy. Confocal excitation maximum = 488 nm, emission maximum = 495 - 595 nm. Two-photon excitation maximum = 976 nm, emission maximum = 475 - 575 nm.

To make a 1000x stock solution, add 200 μL DMSO (Cat. No. 3176).

Optical Data for HBC 530

λ_abs	485 nm
λ_em	530 nm
Extinction Coefficient (ε)	65300 M^-1cm^-1
Quantum Yield (φ)	0.66
Closest Laser line	488 nm
Application	Fluorogen, used with RNA Light-up Aptamer, Confocal Microscopy
Alternative For	Cat. No. 6434

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data for HBC 530

M. Wt	303.37
Formula	C₁₉H₁₇N₃O
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	156840-13-0
InChi Key	DIAVZHWDYXQAFC-LDADJPATSA-N
Smiles	CN(CCO)C1=CC=C(C=C1)/C=C(C#N)/C2=CC=C(C=C2)C#N

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for HBC 530

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	30.34	100

Preparing Stock Solutions for HBC 530

The following data is based on the product molecular weight 303.37.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.30 mL	16.48 mL	32.96 mL
5 mM	0.66 mL	3.30 mL	6.59 mL
10 mM	0.33 mL	1.65 mL	3.30 mL
50 mM	0.07 mL	0.33 mL	0.66 mL

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References for HBC 530

References are publications that support the biological activity of the product.

Chen Visualizing RNA dynamics in live cells with bright and stable fluorescent RNAs. Nat.Biotechnol. 2019 PMID: 31548726