Nucleic Acid Dye Green I
Tocris Bioscience, part of Bio-Techne | Catalog # 7246
Key Product Details
| Description: | High affinity double-stranded DNA (dsDNA) probe; used in RT-LAMP and qPCR |
| Chemical Name: | 2-((2-((3-(Dimethylamino)propyl)(propyl)amino)-1-phenylquinolin-4(1H)-ylidene)methyl)-3-methylbenzo[d]thiazol-3-ium chloride |
| Purity: | ≥95% (HPLC) |
| Molecular Weight: | 545.19 |
Biological Activity for Nucleic Acid Dye Green I
Nucleic Acid Dye Green I is an asymmetric cyanine dye that specifically binds to dsDNA. Frequently used dye in qPCR and is further used as a stain for in-gel visualization of DNA. Also used in the detection of amplification products in Loop-Mediated Isothermal Amplification (LAMP) assays; this method has been used for the detection of SARS-CoV-2 virus. When bound to dsDNA the fluorescence intensity is 1000-fold greater than when in the unbound form. Does not require washing or de-staining steps. Compatible with all commonly used fluorescence detection systems. Excitation maximum = 495 nm; emission maximum = 520 nm.
This product is sold as a DMSO stock.
Usage Information
- Supplied as a 10,000x concentrate in DMSO.
- Soluble in TE (10 mM Tris-HCl, 1 mM EDTA, pH 8.0), TBE, or TAE buffer.
- Verify that the pH of the staining solution, at the temperature used for staining, is between 7.5 and 8.0 (preferably pH 8.0).
- Use diluted samples within 24 hours to ensure maximal sensitivity.
Optical Data for Nucleic Acid Dye Green I
| Emission Color | Green |
|---|---|
| λabs | 495 nm |
| λem | 520 nm |
| Application | Real Time PCR, Nucleic Acid Staining, RT-LAMP |
| Alternative For | SYBR® Green I |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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