AbSelect (TM) Generic Antibody Purification Kit
Novus Biologicals, part of Bio-Techne | Catalog # 860-0010
Key Product Details
Kit Type
Antibody Purification Kit
Product Summary for AbSelect (TM) Generic Antibody Purification Kit
Commercially available antibodies often contain substances (e.g. BSA, glycine, tris, azide) that interfere in labeling reactions. The AbSelect Purification Kit quickly removes these contaminants. It can also be used to purify antibodies from crude samples such as ascites fluid or immune serum.
The antibody to be purified or cleaned up ideally is in a volume of 100 ul to 0.5 ml. Up to 500 ug of antibody can be purified in each run. The AbSelect method involves capture of the antibody on protein A resin and the removal of unwanted substances by a simple wash procedure. The purified product is then eluted and neutralized.
The components of the AbSelect kit are fully compatible with the Lightning-Link antibody labeling system (available separately), which allows the purified antibody to be rapidly conjugated with a wide variety of labels.
The antibody to be purified or cleaned up ideally is in a volume of 100 ul to 0.5 ml. Up to 500 ug of antibody can be purified in each run. The AbSelect method involves capture of the antibody on protein A resin and the removal of unwanted substances by a simple wash procedure. The purified product is then eluted and neutralized.
The components of the AbSelect kit are fully compatible with the Lightning-Link antibody labeling system (available separately), which allows the purified antibody to be rapidly conjugated with a wide variety of labels.
Product Specifications
Application Notes
The volume of antibody to be purified or cleaned up should be 0.1-0.5 mL, though larger volumes may be processed by first incubating the antibody sample (combined with the Binding Buffer) with the Protein A resin in a larger vessel (e.g. 2 mL-eppendorf tube) prior to transferring to the spin cartridge in several aliquots, spinning down the excess liquid each time.
Kit Contents for AbSelect (TM) Generic Antibody Purification Kit
- 1 or 3 spin cartridge / collecting tube assemblies
- 1 or 3 vials of AbSelect Protein A resin
- 1 vial of 10x Binding buffer
- 1 vial of Elution buffer
- 1 vial of Neutralization buffer
- 1 vial of Washing buffer
- 4 or 12 additional collecting tubes
Formulation, Preparation, and Storage
Concentration
Concentration is not relevant for this product. Please see the protocols for proper use of this product.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at 4C.
Background: AbSelect (TM) Generic
Additional AbSelect (TM) Generic Products
Product Documents for AbSelect (TM) Generic Antibody Purification Kit
Product Specific Notices for AbSelect (TM) Generic Antibody Purification Kit
AbSelect® is a registered trademark of Abcam and distributed by Novus Biologicals.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt and this statement overrides any mentioned guarantee period on the limitations section of this products datasheet. Please contact technical@novusbio.com with questions.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.
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