Bovine IgG Fc Isotype Control
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97044
Key Product Details
Species Reactivity
Bovine
Applications
Dot Blot, ELISA, SDS-Page, Western Blot
Label
Unconjugated
Antibody Source
Bovine IgG
Format
Purified
Concentration
Please contact technical services for concentration.
Product Specifications
Specificity
No reaction was observed against anti-Bovine IgG F(ab')2 or anti-Papain.
Host
Bovine
Isotype
IgG
Description
This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Bovine Serum, anti-Bovine IgG and anti-Bovine IgG F(c)
Store vial at 4C prior to opening. This product is stable 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Store vial at 4C prior to opening. This product is stable 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Scientific Data Images for Bovine IgG Fc Isotype Control
Dot Blot: Bovine IgG Fc Isotype Control [NBP1-97044] - Dot Blot of Bovine IgG FcFragment.Lane 1: 100 ng Bovine Fc.Lanes 2-5: serial dilution 3 fold of Antigen.Primary Antibody: Rabbit Anti-Bovine Fc at 1 ug/ml at RT for 1 hour.Secondary Antibody: Rabbit Secondary HRP Antibody at 1:70,000 for 30 min at RT.Block at RT for 30 minutes.
![SDS-PAGE: Bovine IgG Fc Isotype Control [NBP1-97044]](https://resources.bio-techne.com/images/products/Bovine-IgG-Fc-Isotype-Control-SDS-Page-NBP1-97044-img0002.jpg "SDS-PAGE: Bovine IgG Fc Isotype Control [NBP1-97044]")
SDS-PAGE: Bovine IgG Fc Isotype Control [NBP1-97044]
SDS-Page: Bovine IgG Fc Isotype Control [NBP1-97044] - SDS-PAGE for Bovine IgG Fc isotype control.Lane 1: 1ug Reduced Bovine Fc.Lane 2: MW ladder Opal pre-stained.Lane 3: 1ug Non-Reduced Bovine Fc.Coomassie Blue Stained.
Bovine IgG Fc Isotype Control
Dot Blot of Bovine IgG Fc Isotype Control. Lane 1: 100 ng Bovine Fc. Lanes 2-5: serial dilution 3 fold of Antigen. Primary Antibody: Rabbit Anti-Bovine FcApplications for Bovine IgG Fc Isotype Control
Application
Recommended Usage
Dot Blot
Optimal dilutions of this antibody should be experimentally determined.
ELISA
Optimal dilutions of this antibody should be experimentally determined.
SDS-Page
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This product has been tested in SDS-Page and Dot Blot and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
Formulation, Preparation, and Storage
Purification
Multi-step
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Format
Purified
Preservative
0.01% Sodium Azide
Concentration
Please contact technical services for concentration.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
Background: IgG Fc
Additional IgG Fc Products
Product Documents for Bovine IgG Fc Isotype Control
Product Specific Notices for Bovine IgG Fc Isotype Control
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.
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