Mouse IgG3 Isotype Control (MG3)
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-96777
![Western Blot: Mouse IgG3 Isotype Control (MG3) [NBP1-96777]](https://resources.bio-techne.com/images/products/Mouse-IgG3-Isotype-Control-MG3-Western-Blot-NBP1-96777-img0003.jpg "Western Blot: Mouse IgG3 Isotype Control (MG3) [NBP1-96777]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Dot Blot, ELISA, Flow Cytometry, Fluorophore-linked immunosorbent assay, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG3 Clone # MG3
Format
Purified
Concentration
Please contact technical services for concentration.
Product Specifications
Specificity
Isotyping assay resulted non-reactive with antisera to mouse IgG1, IgG2a, IgG2b, IgA, IgM
Clonality
Monoclonal
Host
Mouse
Isotype
IgG3
Description
This antibody has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG3 and anti-Mouse serum. Isotyping assay resulted non-reactive with antisera to mouse IgG1, IgG2a, IgG2b, IgA, IgM. Confirmed by SDS-PAGE.
Store vial at 4C prior to opening. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at 4C prior to opening. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for Mouse IgG3 Isotype Control (MG3)
Western Blot: Mouse IgG3 Isotype Control (MG3) [NBP1-96777]
Western Blot: Mouse IgG3 Isotype Control (MG3) [NBP1-96777] - SDS-PAGE of Mouse IgG3. Lane 1: Non-Reduced Human IgG. Lane 2: 5uL OPAL Pre-stained Marker MB-210-0500. Lane 3: Reduced Mouse IgG3. Load: 1ug per lane. Predicted/Observed size: Non-reduced at 180-245 kDa, Reduced at 55, 25 kDa.
Dot Blot: Mouse IgG3 Isotype Control (MG3) [NBP1-96777] - A three-fold serial dilution of Fluorescein conjugated Mouse IgG3 subclass starting at 200ug was spotted onto 0.45 um nitrocellulose. Image from the FITC version of this antibody.
Applications for Mouse IgG3 Isotype Control (MG3)
Application
Recommended Usage
Flow Cytometry
1:1000 - 1:5000
Immunocytochemistry/ Immunofluorescence
1:10 - 1:500
Western Blot
1:100 - 1:2000
Application Notes
This product has been tested by SDS-Page and ELISA and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Format
Purified
Preservative
0.01% Sodium Azide
Concentration
Please contact technical services for concentration.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IgG3
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Long Name
Immunoglobulin G3
Alternate Names
DKFZp686H11213, FLJ39988, FLJ40036, FLJ40253, FLJ40587, FLJ40789, FLJ40834, IgG3, immunoglobulin heavy constant gamma 3 (G3m marker), MGC45809
Gene Symbol
IGHG3
Additional IgG3 Products
Product Documents for Mouse IgG3 Isotype Control (MG3)
Product Specific Notices for Mouse IgG3 Isotype Control (MG3)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.
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