Human IgM Heavy Chain Isotype Control
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-96772
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry, SDS-Page, Western Blot
Label
Unconjugated
Antibody Source
Human IgM
Format
Purified
Concentration
Please contact technical services for concentration.
Product Summary for Human IgM Heavy Chain Isotype Control
Specificity
No reaction was observed against anti-Trypsin, anti-human IgG F(ab')2, anti-human IgG F(c), anti-human Kappa or anti-human Lambda.
Host
Human
Isotype
IgM
Description
This product has been prepared from Human IgM myeloma protein by digestion with trypsin followed by column chromatography. Purity was assessed by SDS-PAGE and HPLC to be greater than 95%. Assay by immuno-electrophoresis at a concentration of 20 mg/ml
Store vial at 4C prior to opening. This product is stable 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
No test method can provide total assurance that the hepatitis B virus, hepatitis C virus, human immunodeficiency virus, or any other infectious agents are absent. Thus, all blood products, including purified proteins derived from human blood sources, should be handled at Biosafety Level 2 as recommended by the CDC\NIH manual entitled Biosafety in Microbiological and Biomedical Laboratories for potentially infectious human serum, blood specimens or proteins derived from same. Source material for the human blood product supplied to your facility has been tested for the detection of HIV antibody, Hepatitis B surface antigen, antibody to Hepatitis C, HIV 1 antigen(s), antibody to HTLV - I/II, and syphilis by FDA guidelines. All units were found to be non-reactive/negative for these tests. All human blood source material is collected in FDA licensed centers and is tested with FDA approved test kits.
Store vial at 4C prior to opening. This product is stable 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
No test method can provide total assurance that the hepatitis B virus, hepatitis C virus, human immunodeficiency virus, or any other infectious agents are absent. Thus, all blood products, including purified proteins derived from human blood sources, should be handled at Biosafety Level 2 as recommended by the CDC\NIH manual entitled Biosafety in Microbiological and Biomedical Laboratories for potentially infectious human serum, blood specimens or proteins derived from same. Source material for the human blood product supplied to your facility has been tested for the detection of HIV antibody, Hepatitis B surface antigen, antibody to Hepatitis C, HIV 1 antigen(s), antibody to HTLV - I/II, and syphilis by FDA guidelines. All units were found to be non-reactive/negative for these tests. All human blood source material is collected in FDA licensed centers and is tested with FDA approved test kits.
Scientific Data Images for Human IgM Heavy Chain Isotype Control
![SDS-PAGE: Human IgM Heavy Chain Isotype Control [NBP1-96772]](https://resources.bio-techne.com/images/products/Human-IgM-Heavy-Chain-Isotype-Control-SDS-Page-NBP1-96772-img0001.jpg "SDS-PAGE: Human IgM Heavy Chain Isotype Control [NBP1-96772]")
SDS-PAGE: Human IgM Heavy Chain Isotype Control [NBP1-96772]
SDS-Page: Human IgM Heavy Chain Isotype Control [NBP1-96772] - SDS PAGE Results of Human IgM Heavy Chain isotype control. Lane 1: Human IgM Heavy Chain isotype control reduced [5ug]. Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: Human IgM Heavy Chain isotype control non-reduced [5ug]. 4-20% gel, Coomassie stained. Bands at expected MW.Applications for Human IgM Heavy Chain Isotype Control
Application
Recommended Usage
ELISA
1:100 - 1:2000
Immunohistochemistry
1:10 - 1:500
Western Blot
1:100-1:2000
Application Notes
This product has been tested in SDS-Page and can be used in Western Blotting and ELISA experiments as a control reagent.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Multi-step
Formulation
0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
Format
Purified
Preservative
0.05% Sodium Azide
Concentration
Please contact technical services for concentration.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
Background: IgM Heavy Chain
Alternate Names
Constant region of heavy chain of IgM, Ig Mu Chain C Region BOT, Ig Mu Chain C Region GAL, Ig Mu Chain C Region OU, immunoglobulin heavy constant mu, Immunoglobulin mu chain
Gene Symbol
IGHM
Additional IgM Heavy Chain Products
Product Documents for Human IgM Heavy Chain Isotype Control
Product Specific Notices for Human IgM Heavy Chain Isotype Control
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.
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