A-431 Whole Cell Lysate

Human

Western Blot

Ready-to-use A431 Whole Cell Lysate are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOuT dissociating agents . Heat lysate - 95C for 5 minutes and rapidly cool. If dissociating conditions are desired, add reducing agent prior - heating. The recommended loading volume per lane is 10-20 ul depending on the size format of your gel.

A431 whole cell extract lysate

Cell

Skin

Whole

1X SDS-PAGE Sample Buffer [0.05 mM Tris HCl, 2% SDS, 10% Glycerol, 0.1% bromophenol blue, pH 6.8]

1 mg/ml

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Store at -80C. Avoid freeze-thaw cycles.