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A-431 Whole Cell Lysate

Novus Biologicals, part of Bio-Techne | Catalog # NB820-59461

Novus Biologicals, part of Bio-Techne
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NB820-59461

Key Product Details

Species

Human

Applications

Western Blot

Product Summary for A-431 Whole Cell Lysate

We recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use.

Product Specifications

Application Notes

Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression

Specificity

Total Protein - Human Tumor Cell Line: A431

Type

Cell

Tissue

Skin

Protein State

Native

Subcellular Fraction

Whole

Formulation, Preparation, and Storage

Formulation

HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors

Concentration

5.0 mg/ml

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -80C. Avoid freeze-thaw cycles.

Background: A-431

Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.

Additional A-431 Products

Product Documents for A-431 Whole Cell Lysate

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for A-431 Whole Cell Lysate

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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