Mouse Brain Whole Tissue Lysate (Adult Whole Normal)
Novus Biologicals, part of Bio-Techne | Catalog # NB820-59657
Key Product Details
Species
Mouse
Applications
Immunoprecipitation, SDS-PAGE, Western Blot
Product Summary for Mouse Brain Whole Tissue Lysate (Adult Whole Normal)
We recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use.
Product Specifications
Applications
Western Blot (10 ug- 20 ug)
Application Notes
Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression
Specificity
Total Protein - Mouse Normal Tissue: Brain
Type
Tissue
Life Stage
Adult
Tissue
Brain
Protein State
Native
Tissue Condition
Normal
Subcellular Fraction
Whole
Scientific Data Images for Mouse Brain Whole Tissue Lysate (Adult Whole Normal)
![Western Blot: Mouse Brain Whole Tissue Lysate (Adult Whole Normal) [NB820-59657]](https://resources.bio-techne.com/images/products/Mouse-Brain-Whole-Tissue-Lysate-Adult-Normal-Western-Blot-NB820-59657-img0005.jpg "Western Blot: Mouse Brain Whole Tissue Lysate (Adult Whole Normal) [NB820-59657]")
Western Blot: Mouse Brain Whole Tissue Lysate (Adult Whole Normal) [NB820-59657]
Western Blot: Mouse Brain Whole Tissue Lysate (Adult Normal) [NB820-59657] - Western blot analysis of GAPDH (NB300-323) using whole normal mouse brain lysate [NB820-59657].![SDS-PAGE: Mouse Brain Whole Tissue Lysate (Adult Whole Normal) [NB820-59657]](https://resources.bio-techne.com/images/products/Mouse-Brain-Whole-Tissue-Lysate-Adult-Normal-SDS-Page-NB820-59657-img0004.jpg "SDS-PAGE: Mouse Brain Whole Tissue Lysate (Adult Whole Normal) [NB820-59657]")
SDS-PAGE: Mouse Brain Whole Tissue Lysate (Adult Whole Normal) [NB820-59657]
SDS-Page: Mouse Brain Whole Tissue Lysate (Adult Normal) [NB820-59657] - Ponceau S staining of whole normal mouse brain lysate [NB820-59657].![SDS-PAGE: Mouse Brain Whole Tissue Lysate (Adult Whole Normal) [NB820-59657]](https://resources.bio-techne.com/images/products/Mouse-Brain-Whole-Tissue-Lysate-Whole-Normal-SDS-Page-NB820-59657-img0006.jpg "SDS-PAGE: Mouse Brain Whole Tissue Lysate (Adult Whole Normal) [NB820-59657]")
SDS-PAGE: Mouse Brain Whole Tissue Lysate (Adult Whole Normal) [NB820-59657]
SDS-Page: Mouse Brain Whole Tissue Lysate (Whole Normal) [NB820-59657] - Protein lysate is positively confirmed by Western Blot using specific biomarker: GAPDH for total protein, cytoplasmic and universal total protein; Histone for nuclear protein; Na/K ATPase for membrane protein; Vimentin for cytoskeleton protein.Formulation, Preparation, and Storage
Formulation
HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors
Concentration
5 mg/ml
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at -80C. Avoid freeze-thaw cycles.
Background: Brain
Additional Brain Products
Product Documents for Mouse Brain Whole Tissue Lysate (Adult Whole Normal)
Product Specific Notices for Mouse Brain Whole Tissue Lysate (Adult Whole Normal)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.
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