CCR5 Overexpression Lysate
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-09009
Key Product Details
Species
Applications
Product Summary for CCR5 Overexpression Lysate
Expression Host: HEK293T
Plasmid: RC223291
Accession#: NM_000579
Protein Tag: C-MYC/DDK
You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Product Specifications
Application Notes
Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
TMW
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Type
Scientific Data Images for CCR5 Overexpression Lysate
Western Blot: CCR5 Overexpression Lysate [NBP2-09009]
Western Blot: CCR5 Overexpression Lysate (Adult Normal) [NBP2-09009] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CCR5.Formulation, Preparation, and Storage
Formulation
Concentration
Shipping
Storage
Background: CCR5
CCR5 is a major coreceptor for human HIV infection and is predominantly expressed on T cells, macrophages, dendritic cells and microglia. It is likely that CCR5 plays a role in inflammatory responses to infection, though its exact role in normal immune function is unclear.
CCR5 antibodies are useful for HIV infection studies and research on immune responses.
Alternate Names
Gene Symbol
Additional CCR5 Products
Product Documents for CCR5 Overexpression Lysate
Product Specific Notices for CCR5 Overexpression Lysate
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.