CENPB 293T Cell Transient Overexpression Lysate, Novus Biologicals

Name: CENPB 293T Cell Transient Overexpression Lysate
Brand: Novus Biologicals
SKU: H00001059-T01

Bio-Techne includes Novus Biologicals | Catalog # H00001059-T01

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Product Datasheet / COA

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Product Summary
Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species

Human

Applications

Western Blot

Product Summary for CENPB 293T Cell Transient Overexpression Lysate

Quality control test: Transient overexpression cell lysate was tested with Anti-CENPB antibody by Western Blots. Plasmid: pCMV-CENPB full-length

Product Specifications

Specificity

CENPB 293T Cell Transient Overexpression Lysate(Denatured)

TMW

80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Type

293T Cell Transient Overexpression

Protein State

Denatured

Scientific Data Images for CENPB 293T Cell Transient Overexpression Lysate

CENPB transfected lysate.

Lane 1: CENPB transfected lysate (66 KDa) Lane 2: Non-transfected lysate.

Formulation, Preparation, and Storage

Formulation

1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)

Concentration

mg/ml

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -80C. Avoid freeze-thaw cycles.

Background: CENPB

This gene product is a highly conserved protein that facilitates centromere formation. It is a DNA-binding protein that is derived from transposases of the pogo DNA transposon family. It contains a helix-loop-helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. The DNA binding domain recognizes and binds a 17-bp sequence (CENP-B box) in the centromeric alpha satellite DNA. This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes. It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies. [provided by RefSeq]

Alternate Names

CENP-B, centromere autoantigen B, Centromere protein B, centromere protein B (80kD), centromere protein B, 80kDa, major centromere autoantigen B

Gene Symbol

CENPB

Additional CENPB Products

Product Documents for CENPB 293T Cell Transient Overexpression Lysate

Product Datasheets

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COA

Product Specific Notices for CENPB 293T Cell Transient Overexpression Lysate

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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