COL4A3BP Overexpression Lysate

Name: COL4A3BP Overexpression Lysate
Brand: Novus Biologicals
SKU: NBL1-09354

Novus Biologicals, part of Bio-Techne | Catalog # NBL1-09354

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Product Summary
Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species

Human

Applications

Western Blot

Product Summary for COL4A3BP Overexpression Lysate

COL4A3BP Transient Overexpression Lysate
Expression Host: HEK293T

Plasmid: RC200316

Accession#: NM_031361

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.

Product Specifications

Application Notes

This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.

TMW

67.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Type

Overexpression

Scientific Data Images for COL4A3BP Overexpression Lysate

Western Blot: COL4A3BP Overexpression Lysate [NBL1-09354]

Western Blot: COL4A3BP Overexpression Lysate (Adult Normal) [NBL1-09354] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for COL4A3BP.

Formulation, Preparation, and Storage

Formulation

RIPA buffer

Concentration

The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -80C. Avoid freeze-thaw cycles.

Background: COL4A3BP

This gene encodes a kinase that specifically phosphorylates the N-terminal region of the non-collagenous domain of the alpha 3 chain of type IV collagen, known as the Goodpasture antigen. Goodpasture disease is the result of an autoimmune response directed at this antigen. One isoform of this protein is also involved in ceramide intracellular transport. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (2) differs in the 5' UTR and coding region and lacks an alternate in-frame exon compared to variant 3. The resulting isoform (2) is shorter at the N-terminus and lacks an internal segment compared to isoform 3. The absence of the in-frame exon results in the loss of a protein motif and less phosphorylation activity. This isoform also mediates intracellular transport of ceramide from the endoplasmic reticulum to the Golgi apparatus. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Alternate Names

Ceramide transfer protein, CERTFLJ20597, CERTL, collagen type IV alpha-3-binding protein, collagen, type IV, alpha 3 (Goodpasture antigen) binding protein, Goodpasture antigen-binding protein, GPBPSTARD11ceramide transporter, hCERT, lipid-transfer protein CERTL, StARD11, StAR-related lipid transfer (START) domain containing 11, StAR-related lipid transfer protein 11, START domain containing 11, START domain-containing protein 11

Gene Symbol

CERT1

Additional COL4A3BP Products

Product Documents for COL4A3BP Overexpression Lysate

Product Datasheets

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COA

SDS

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Product Specific Notices for COL4A3BP Overexpression Lysate

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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