Colon Matched Tumor and Normal Tissue Lysate
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47078
Key Product Details
Species
Human
Product Summary for Colon Matched Tumor and Normal Tissue Lysate
1 x Human colon tumor tissue lysate (1 mg/ml, 100 ug/vial)
1 x Human colon normal tissue lysate (matched) (1 mg/ml, 100 ug/vial)
Matched Tumor & Normal Tissue Lysate SetDiagnosis: Adenocarcinoma
Sex: Female
Age: 46
Grade: 1
Stage: II, T3N0M0
Tumor Pathology Data
Location: Rectum
Gross findings: Tumor size 4 x 3 x 2 cm. Cut surface is soft, white. Regional lymph nodes: 3
Microscopic Findings
Tissue sections show proliferation of malignant epithelial glands. Epithelial cells have small, basophilic, irregular nuclei and abundant cytoplasm. Cytoplasm contains muscin. Tumor cells arranged in glands. Mitotic figures are not evident. The tumor cells invaded the muscularis propria and subserosa. The stroma is infiltrated by large numbers of inflammatory cells. Tumor necrosis and blood vessel invasion are not revealed. Lymph nodes show hyperplasia.
Preparation Method
Tissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify.
Extraction 1: PBS, pH 7.4; 1 ug/ml Aprotinin; 1 mM NaF
Modified RIPA Buffer: 1 mM EDTA; 1 ug/ml Pepstatin-A; 0.1% SDS; 0.25% Na deoxycholate; 1 ug/ml Leupeptin; 1 mM PMSF; 1 mM Na3VO4
1 x Human colon normal tissue lysate (matched) (1 mg/ml, 100 ug/vial)
Matched Tumor & Normal Tissue Lysate SetDiagnosis: Adenocarcinoma
Sex: Female
Age: 46
Grade: 1
Stage: II, T3N0M0
Tumor Pathology Data
Location: Rectum
Gross findings: Tumor size 4 x 3 x 2 cm. Cut surface is soft, white. Regional lymph nodes: 3
Microscopic Findings
Tissue sections show proliferation of malignant epithelial glands. Epithelial cells have small, basophilic, irregular nuclei and abundant cytoplasm. Cytoplasm contains muscin. Tumor cells arranged in glands. Mitotic figures are not evident. The tumor cells invaded the muscularis propria and subserosa. The stroma is infiltrated by large numbers of inflammatory cells. Tumor necrosis and blood vessel invasion are not revealed. Lymph nodes show hyperplasia.
Preparation Method
Tissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify.
Extraction 1: PBS, pH 7.4; 1 ug/ml Aprotinin; 1 mM NaF
Modified RIPA Buffer: 1 mM EDTA; 1 ug/ml Pepstatin-A; 0.1% SDS; 0.25% Na deoxycholate; 1 ug/ml Leupeptin; 1 mM PMSF; 1 mM Na3VO4
Product Specifications
Application Notes
These lysates have not been subjected to denaturing or reducing conditions. This allows the tissue or cell lysate to be used in a variety of applications; to study protein-protein interaction, ligand binding, ELISA, immunoprecipitation, 1D and 2D gel electrophoresis, and Western blotting for the detection of specific protein targets.
For use in 1D and 2D gel electrophoresis, the addition of a denaturing gel loading buffer with reducing agents may be required.
Buffer requirements for performing protein-protein interaction and ligand binding studies can vary significantly from RIPA buffer and may require modifications. In most cases, tissue lysates in RIPA buffer can be used, directly in standard ELISA and immunoprecipitation assays.
These lysates are proteomic discovery tools. Researchers should validate and optimize for individual use.
For use in 1D and 2D gel electrophoresis, the addition of a denaturing gel loading buffer with reducing agents may be required.
Buffer requirements for performing protein-protein interaction and ligand binding studies can vary significantly from RIPA buffer and may require modifications. In most cases, tissue lysates in RIPA buffer can be used, directly in standard ELISA and immunoprecipitation assays.
These lysates are proteomic discovery tools. Researchers should validate and optimize for individual use.
Type
Matched Tumor and Normal Tissue
Life Stage
Adult
Tissue
Colon
Formulation, Preparation, and Storage
Concentration
mg/ml
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at -80C. Avoid freeze-thaw cycles.
Background: Colon
Additional Colon Products
Product Documents for Colon Matched Tumor and Normal Tissue Lysate
Product Specific Notices for Colon Matched Tumor and Normal Tissue Lysate
The vial is provided with a 10% overfill. Maximum recovery can be obtained by centrifuging the vial briefly to collect any solution on the cap and tube sides.
This material has tested negative for HbsAg, HIV 1/2, and HCV. Use UNIVERSAL PRECAUTIONS when handling. Human tissue derivatives must be treated as a potentially infectious agent and disposed of appropriately
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.
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