Dicer knockout Mouse embryonic stem cells Lysate

Mouse

Immunoprecipitation, Western Blot

This lysate is prepared as a positive control for immunoprecipitation or separation by SDS-PAGE and subsequent Western blot analysis. Lysates are prepared in denaturing buffer WITHOUT loading buffer or reducing agents (i.e. 2-mercaptoethanol (BME) or dithiothreitol (DTT)). If reducing conditions are desired, add a reducing agent prior to heating. Heat lysate to 95 degrees C for 5 minutes and rapidly cool. The recommended loading amount is 0.01-0.03 mg per lane.

Embryo

Whole

Standard RIPA buffer including protease and phosphatase inhibitors and 0.1% SDS. No reducing agents or loading buffer added.

No Preservative

Please contact technical services for concentration.

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Store at -80C. Avoid freeze-thaw cycles.