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FCGR2C Overexpression Lysate

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-04598

Novus Biologicals, part of Bio-Techne
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NBP2-04598

Key Product Details

Species

Human

Applications

Western Blot

Product Summary for FCGR2C Overexpression Lysate

FCGR2C Transient Overexpression Lysate
Expression Host: HEK293T

Plasmid: RC216432

Accession#: NM_201563

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.

Product Specifications

Application Notes

This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.

TMW

35.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Type

Overexpression

Scientific Data Images for FCGR2C Overexpression Lysate

Western Blot: FCGR2C Overexpression Lysate [NBP2-04598]

Western Blot: FCGR2C Overexpression Lysate [NBP2-04598]

Western Blot: FCGR2C Overexpression Lysate (Adult Normal) [NBP2-04598] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FCGR2C.

Formulation, Preparation, and Storage

Formulation

RIPA buffer

Concentration

The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -80C. Avoid freeze-thaw cycles.

Background: Fc gamma RIIC/CD32c

The FCGR2C gene encodes one of three members of a family of low-affinity immunoglobulin gamma Fc receptors found on the surface of many immune response cells. The encoded protein is a transmembrane glycoprotein and may be involved in phagocytosis and clearing o

Long Name

Fc gamma Receptor II

Alternate Names

CD32c, FCGR2C, FcgRIIC, FCRIIC

Gene Symbol

FCGR2C

Additional Fc gamma RIIC/CD32c Products

Product Documents for FCGR2C Overexpression Lysate

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for FCGR2C Overexpression Lysate

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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