Jurkat Staurosporine Treated / Untreated Cell Lysate

SDS-PAGE

SDS-Page (10 ul / lane)

Jurkat whole cell lysate is provided as a Western blot positive control. The lysates are provided as three vials of samples with varying treatment times. Use 10 ul per lane for a standard mini-gel blot (1.0 mg/ml).

Cell

Staurosporine Treated / Untreated

Jurkat cells are grown under standard tissue culture conditions until semi-confluent (70-80%). The cells are then treated with 1 uM Staurosporine for 0, 1 and 4 hours. The cells are then washed in PBS and directly lysed into 1X Laemmli sample buffer with BME. The lysate is sonicated and tested by Western blot.

The protein lysate is prepared in 1x Laemmli sample buffer with BME. Boiling before loading is not necessary.

1.0 mg/ml

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Store at -20 to -80C. Avoid freeze-thaw cycles.