KIR2DL5/CD158f Overexpression Lysate

Name: KIR2DL5/CD158f Overexpression Lysate
Brand: Novus Biologicals
SKU: NBP2-06630

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-06630

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Product Summary
Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species

Human

Applications

Western Blot

Product Summary for KIR2DL5/CD158f Overexpression Lysate

KIR2DL5/CD158f Transient Overexpression Lysate
Expression Host: HEK293T

Plasmid: RC217119

Accession#: NM_020535

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.

Product Specifications

Application Notes

This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.

TMW

40.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Type

Overexpression

Scientific Data Images for KIR2DL5/CD158f Overexpression Lysate

Western Blot: KIR2DL5/CD158f Overexpression Lysate [NBP2-06630]

Western Blot: KIR2DL5A Overexpression Lysate (Adult Normal) [NBP2-06630] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for KIR2DL5A.

Formulation, Preparation, and Storage

Formulation

RIPA buffer

Concentration

The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -80C. Avoid freeze-thaw cycles.

Background: KIR2DL5/CD158f

Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by natural killer cells andsubsets of T cells. The KIR genes are polymorphic and highly homologous and they are found in a cluster on chromosome19q13.4 within the 1 Mb leukocyte receptor complex (LRC). The gene content of the KIR gene cluster varies amonghaplotypes, although several "framework" genes are found in all haplotypes (KIR3DL3, KIR3DP1, KIR3DL4,KIR3DL2). The KIR proteins are classified by the number of extracellular immunoglobulin domains (2D or 3D) and bywhether they have a long (L) or short (S) cytoplasmic domain. KIR proteins with the long cytoplasmic domain transduceinhibitory signals upon ligand binding via an immune tyrosine-based inhibitory motif (ITIM), while KIR proteins withthe short cytoplasmic domain lack the ITIM motif and instead associate with the TYRO protein tyrosine kinase bindingprotein to transduce activating signals. The ligands for several KIR proteins are subsets of HLA class I molecules;thus, KIR proteins are thought to play an important role in regulation of the immune response. (provided by RefSeq)

Long Name

Killer Cell Immunoglobulin-like Receptor, Two Domain Long Cytoplasmic Tail, 5

Alternate Names

CD158f, KIR2DL5A

Gene Symbol

KIR2DL5A

Additional KIR2DL5/CD158f Products

Product Documents for KIR2DL5/CD158f Overexpression Lysate

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for KIR2DL5/CD158f Overexpression Lysate

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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