LRRN1/NLRR-1 Overexpression Lysate
Novus Biologicals, part of Bio-Techne | Catalog # NBL1-12711
Key Product Details
Species
Human
Applications
Western Blot
Product Summary for LRRN1/NLRR-1 Overexpression Lysate
LRRN1/NLRR-1 Transient Overexpression Lysate
Expression Host: HEK293T
Plasmid: RC205198
Accession#: NM_020873
Protein Tag: C-MYC/DDK
You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Expression Host: HEK293T
Plasmid: RC205198
Accession#: NM_020873
Protein Tag: C-MYC/DDK
You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Product Specifications
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.
Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
TMW
80.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Type
Overexpression
Scientific Data Images for LRRN1/NLRR-1 Overexpression Lysate
![Western Blot: LRRN1/NLRR-1 Overexpression Lysate [NBL1-12711]](https://resources.bio-techne.com/images/products/LRRN1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12711-img0002.jpg "Western Blot: LRRN1/NLRR-1 Overexpression Lysate [NBL1-12711]")
Western Blot: LRRN1/NLRR-1 Overexpression Lysate [NBL1-12711]
Western Blot: LRRN1 Overexpression Lysate (Adult Normal) [NBL1-12711] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for LRRN1.Formulation, Preparation, and Storage
Formulation
RIPA buffer
Concentration
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at -80C. Avoid freeze-thaw cycles.
Background: LRRN1/NLRR-1
LRRN1 (Leucine-rich repeat neuronal protein 1; NLRR1) is a 95 kDa member of the neuronal LRR family of proteins. It is a cell-surface glycoprotein that is expressed on embryonic motor neurons and somatic myoblasts. Human LRRN1 is 716 amino acids (aa) in length. It is a type I transmembrane protein that contains a 606 aa extracellular region (aa 26 - 631) and a short 64 aa cytoplasmic tail. The extracellular region shows eleven LRRs (aa 94 - 384) that mediate protein-protein interactions, one C2-type Ig-like domain and one fibronectin type III domain. There is one potential alternate start site at Met286. Over aa 26 - 631, human LRRN1 shares 96% aa identity with mouse LRRN1.
Long Name
Leucine-rich Repeat Neuronal 1
Alternate Names
FIGLER3, LRN, NLRR-1, NLRR1
Gene Symbol
LRRN1
Additional LRRN1/NLRR-1 Products
Product Documents for LRRN1/NLRR-1 Overexpression Lysate
Product Specific Notices for LRRN1/NLRR-1 Overexpression Lysate
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.
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