I purchased your product Presenilin 1 Lysate (NBL1-14860) this year. I would like to know if you could tell me the exact concentration of Presenilin-1 present in such lysate, since the concentration given (1mg/ml) is for the total protein content. It would be really helpful for our experiments ongoing at the moment regarding AD.

Name: Presenilin-1 Overexpression Lysate
Brand: Novus Biologicals
SKU: NBL1-14860

The Presenilin 1 lysate was produced in HEK293T cells using plasmid ID RC216443. The concentration of the lysate protein is 1mg/ml however, I'm unable to confirm the exact amount or concentration of the overexpressed protein. The protein contains a C-terminal DDK tag and its presence in the lysate was confirmed by Western blot.

Presenilin-1 Overexpression Lysate

Novus Biologicals, part of Bio-Techne | Catalog # NBL1-14860

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Product Summary
Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species

Human

Applications

Western Blot

Product Summary for Presenilin-1 Overexpression Lysate

Presenilin-1 Transient Overexpression Lysate
Expression Host: HEK293T

Plasmid: RC216443

Accession#: NM_000021

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.

Product Specifications

Application Notes

This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.

TMW

52.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Type

Overexpression

Scientific Data Images for Presenilin-1 Overexpression Lysate

Western Blot: Presenilin-1 Overexpression Lysate [NBL1-14860]

Western Blot: Presenilin 1 Overexpression Lysate (Adult Normal) [NBL1-14860] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Presenilin 1.

Formulation, Preparation, and Storage

Formulation

RIPA buffer

Concentration

The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -80C. Avoid freeze-thaw cycles.

Background: Presenilin-1

Alzheimer's disease (AD) patients with an inherited form of the disease carry mutations in the presenilin proteins (PSEN1; PSEN2) or in the amyloid precursor protein (APP). These disease-linked mutations result in increased production of the longer form of amyloid-beta (main component of amyloid deposits found in AD brains). Presenilins are postulated to regulate APP processing through their effects on gamma-secretase, an enzyme that cleaves APP. Also, it is thought that the presenilins are involved in the cleavage of the Notch receptor, such that they either directly regulate gamma-secretase activity or themselves are protease enzymes. Several alternatively spliced transcript variants encoding different isoforms have been identified for this gene, the full-length nature of only some have been determined. [provided by RefSeq]. Transcript Variant: This variant (1) encodes the longer isoform (I-467). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Alternate Names

Presenilin1, PSEN1, S182

Gene Symbol

PSEN1

Additional Presenilin-1 Products

Product Documents for Presenilin-1 Overexpression Lysate

Product Datasheets

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COA

SDS

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Product Specific Notices for Presenilin-1 Overexpression Lysate

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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