Pyruvate Dehydrogenase Phosphatase/PDP1/PPM2C Overexpression Lysate

Name: Pyruvate Dehydrogenase Phosphatase/PDP1/PPM2C Overexpression Lysate
Brand: Novus Biologicals
SKU: NBP2-11477

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-11477

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Product Summary
Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species

Human

Applications

Western Blot

Product Summary for Pyruvate Dehydrogenase Phosphatase/PDP1/PPM2C Overexpression Lysate

PDP1/PPAPDC2 Transient Overexpression Lysate
Expression Host: HEK293T

Plasmid: RC228947

Accession#: NM_001161781

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.

Product Specifications

Application Notes

This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.

TMW

60.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Type

Overexpression

Scientific Data Images for Pyruvate Dehydrogenase Phosphatase/PDP1/PPM2C Overexpression Lysate

Western Blot: Pyruvate Dehydrogenase Phosphatase/PDP1/PPM2C Overexpression Lysate [NBP2-11477]

Western Blot: Pyruvate Dehydrogenase Phosphatase/PDP1/PPM2C Overexpression Lysate [NBP2-11477] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Pyruvate Dehydrogenase Phosphatase/PDP1/PPM2C.

Formulation, Preparation, and Storage

Formulation

RIPA buffer

Concentration

The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -80C. Avoid freeze-thaw cycles.

Background: Pyruvate Dehydrogenase Phosphatase/PDP1

Pyruvate dehydrogenase (E1) is one of the three components (E1, E2, and E3) of the large pyruvate dehydrogenase complex. Pyruvate dehydrogenase kinases catalyze phosphorylation of serine residues of E1 to inactivate the E1 component and inhibit the complex. Pyruvate dehydrogenase phosphatases catalyze the dephosphorylation and activation of the E1 component to reverse the effects of pyruvate dehydrogenase kinases. Pyruvate dehydrogenase phosphatase is a heterodimer consisting of catalytic and regulatory subunits. Two catalytic subunits have been reported; one is predominantly expressed in skeletal muscle and another one is is much more abundant in the liver. The catalytic subunit, encoded by this gene, is the former, and belongs to the protein phosphatase 2C (PP2C) superfamily. Along with the pyruvate dehydrogenase complex and pyruvate dehydrogenase kinases, this enzyme is located in the mitochondrial matrix. Mutation in this gene causes pyruvate dehydrogenase phosphatase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.(provided by RefSeq)

Alternate Names

PDH, PDP1, PDPC 1, PPM2C

Gene Symbol

PDP1

UniProt

Q9P0J1 (Human), A0A024R9C0 (Human)

Additional Pyruvate Dehydrogenase Phosphatase/PDP1 Products

Product Documents for Pyruvate Dehydrogenase Phosphatase/PDP1/PPM2C Overexpression Lysate

Product Datasheets

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COA

SDS

Download SDS

Product Specific Notices for Pyruvate Dehydrogenase Phosphatase/PDP1/PPM2C Overexpression Lysate

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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