Raw 264.7 Whole Cell Lysate

Name: Raw 264.7 Whole Cell Lysate
Brand: Novus Biologicals
SKU: NBP2-10266

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-10266

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Product Datasheet / COA

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Product Summary
Product Specifications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species

Mouse

Applications

Western Blot

Product Summary for Raw 264.7 Whole Cell Lysate

GROWTH MEDIA: DMEM & 10% FBS (fetal bovine serum)

Product Specifications

Specificity

RAW 264.7 (Abelson Murine Leukemia virus-induced tumor)

Type

Cell

Tissue

Blood Lymph

Tissue Condition

Abelson Leukemia virus transformed

Subcellular Fraction

Whole

Formulation, Preparation, and Storage

Formulation

SDS sample buffer containing 50 mM DTT.

Concentration

2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at 4C short term. Aliquot and store at -70C long term. Avoid freeze-thaw cycles.

Background: Raw 264.7

RAW 264.7 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 ug/ml of aprotinin, 5 ug/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The RAW 264.7 lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Additional Raw 264.7 Products

Product Documents for Raw 264.7 Whole Cell Lysate

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COA

Product Specific Notices for Raw 264.7 Whole Cell Lysate

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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