Raw 264.7 Hypoxic / Normoxic Cell Lysate
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11860
Key Product Details
Applications
SDS-PAGE
Product Specifications
Applications
SDS-Page (10 ul / lane)
Application Notes
RAW264.7 whole cell lysate is provided as a Western blot positive control. The lysates are provided as a set of treated and untreated samples. Use 10 ul per lane for a standard mini-gel blot (1.0 mg/ml).
Type
Cell
Subcellular Fraction
Hypoxic / Normoxic
Formulation, Preparation, and Storage
Preparation Method
RAW264.7 cells are grown under standard tissue culture conditions until semi-confluent (70-80%). The cells are either grown under normoxic conditions or true hypoxic conditions where the oxygen level in the incubator is reduced to 2% for 4 hours. The cells are then washed in PBS and directly lysed into 1X Laemmli sample buffer with BME. The lysate is sonicated and tested by Western blot
Formulation
The protein lysate is prepared in 1x Laemmli sample buffer with BME. Boiling before loading is not necessary.
Concentration
1.0 mg/ml
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Raw 264.7
Additional Raw 264.7 Products
Product Documents for Raw 264.7 Hypoxic / Normoxic Cell Lysate
Product Specific Notices for Raw 264.7 Hypoxic / Normoxic Cell Lysate
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.
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