RBMY1A1 Overexpression Lysate

Name: RBMY1A1 Overexpression Lysate
Brand: Novus Biologicals
SKU: NBL1-15224

Novus Biologicals, part of Bio-Techne | Catalog # NBL1-15224

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Product Summary
Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species

Human

Applications

Western Blot

Product Summary for RBMY1A1 Overexpression Lysate

RBMY1A1 Transient Overexpression Lysate
Expression Host: HEK293T

Plasmid: RC208402

Accession#: NM_001007526

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.

Product Specifications

Application Notes

This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.

TMW

40.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Type

Overexpression

Scientific Data Images for RBMY1A1 Overexpression Lysate

Western Blot: RBMY1A1 Overexpression Lysate [NBL1-15224]

Western Blot: RBMY1A1 Overexpression Lysate (Adult Normal) [NBL1-15224] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RBMY1A1.

Formulation, Preparation, and Storage

Formulation

RIPA buffer

Concentration

The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -80C. Avoid freeze-thaw cycles.

Background: RBMY1A1

This gene encodes a protein containing an RNA-binding motif in the N-terminus and four SRGY (serine, arginine, glycine, tyrosine) boxes in the C-terminus. Multiple copies of this gene are found in the AZFb azoospermia factor region of chromosome Y and the encoded protein is thought to be involved in spermatogenesis. Most copies of this locus are pseudogenes, although six highly similar copies have full-length ORFs and are considered functional. Four functional copies of this gene are found within inverted repeat IR2; two functional copies of this gene are found in palindrome P3, along with two copies of PTPN13-like, Y-linked. Alternative splicing of transcripts results in two transcript variants that encode different proteins. Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 1. Variant 2 encodes isoform 2, which lacks an internal segment, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Alternate Names

MGC181956, RBM, RBM1, RBM2, RBMY, RBMY1C, RNA binding motif protein, Y chromosome, family 1, member A1, RNA binding motif protein, Y-linked, family 1, member A1, RNA binding protein, RNA-binding motif protein 1, RNA-binding motif protein 2, RNA-binding motif protein, Y chromosome, family 1 member A1/C, y chromosome RNA recognition motif 1, YRRM1, YRRM2

Gene Symbol

RBMY1A1

Additional RBMY1A1 Products

Product Documents for RBMY1A1 Overexpression Lysate

Product Datasheets

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COA

SDS

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Product Specific Notices for RBMY1A1 Overexpression Lysate

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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