Rat Skin Whole Tissue Lysate (Adult Whole Normal)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-10273

Novus Biologicals, part of Bio-Techne
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NBP2-10273-0.1mg
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Key Product Details

Species

Rat

Applications

Western Blot

Product Specifications

Specificity

Rat skin tissue from outbred Sprague Dawley Albino rat

Type

Tissue

Life Stage

Adult

Tissue

Skin

Protein State

Native

Tissue Condition

Normal

Subcellular Fraction

Whole

Formulation, Preparation, and Storage

Formulation

SDS sample buffer containing 50 mM DTT.

Concentration

2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at 4C short term. Aliquot and store at -70C long term. Avoid freeze-thaw cycles.

Background: Skin

Skin tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 ug/ml of aprotinin, 5 ug/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Additional Skin Products

Product Documents for Rat Skin Whole Tissue Lysate (Adult Whole Normal)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Rat Skin Whole Tissue Lysate (Adult Whole Normal)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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