Thrombopoietin R/Tpo R 293T Cell Transient Overexpression Lysate, Novus Biologicals

Bio-Techne includes Novus Biologicals | Catalog # H00004352-T01

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Key Product Details

Species

Human

Applications

Western Blot

Product Summary for Thrombopoietin R/Tpo R 293T Cell Transient Overexpression Lysate

Quality control test: Transient overexpression cell lysate was tested with Anti-MPL antibody by Western Blots. Plasmid: pCMV-MPL full-length

Product Specifications

Specificity

Thrombopoietin R/Tpo R 293T Cell Transient Overexpression Lysate(Denatured)

Type

293T Cell Transient Overexpression

Protein State

Denatured

Scientific Data Images for Thrombopoietin R/Tpo R 293T Cell Transient Overexpression Lysate

MPL transfected lysate.
Lane 1: MPL transfected lysate (69.96 KDa) Lane 2: Non-transfected lysate.

Formulation, Preparation, and Storage

Formulation

1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)

Concentration

mg/ml

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -80C. Avoid freeze-thaw cycles.

Background: Thrombopoietin R/Tpo R

In 1990 an oncogene, v-mpl, was identified from the murine myeloproliferative leukemia virus that was capable of immortalizing bone marrow hematopoietic cells from different lineages. In 1992 the human homologue, named, c-mpl, was cloned. Sequence data revealed that c-mpl encoded a protein that was homologous with members of the hematopoietic receptor superfamily. Presence of anti-sense oligodeoxynucleotides of c-mpl inhibited megakaryocyte colony formation. The ligand for c-mpl, thrombopoietin, was cloned in 1994. Thrombopoietin was shown to be the major regulator of megakaryocytopoiesis and platelet formation. The protein encoded by the c-mpl gene, CD110, is a 635 amino acid transmembrane domain, with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs . TPO-R deficient mice were severely thrombocytopenic, emphasizing the important role of CD110 and thrombopoietin in megakaryocyte and platelet formation. Upon binding of thrombopoietin CD110 is dimerized and the JAK family of non-receptor tyrosine kinases, as well as the STAT family, the MAPK family, the adaptor protein Shc and the receptors themselves become tyrosine phosphorylated. [provided by RefSeq]

Long Name

Thrombopoietin Receptor

Alternate Names

c-Mpl, CD110, MPL, MPLV, TpoR

Gene Symbol

MPL

Additional Thrombopoietin R/Tpo R Products

Product Documents for Thrombopoietin R/Tpo R 293T Cell Transient Overexpression Lysate

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Thrombopoietin R/Tpo R 293T Cell Transient Overexpression Lysate

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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