TMPRSS4 Overexpression Lysate
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-04784
Key Product Details
Species
Human
Applications
Western Blot
Product Summary for TMPRSS4 Overexpression Lysate
TMPRSS4 Transient Overexpression Lysate
Expression Host: HEK293T
Plasmid: RC202384
Accession#: NM_183247
Protein Tag: C-MYC/DDK
You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Expression Host: HEK293T
Plasmid: RC202384
Accession#: NM_183247
Protein Tag: C-MYC/DDK
You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Product Specifications
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.
Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Type
Overexpression
Scientific Data Images for TMPRSS4 Overexpression Lysate
![Western Blot: TMPRSS4 Overexpression Lysate [NBP2-04784]](https://resources.bio-techne.com/images/products/TMPRSS4-Overexpression-Lysate-Native-Western-Blot-NBP2-04784-img0002.jpg "Western Blot: TMPRSS4 Overexpression Lysate [NBP2-04784]")
Western Blot: TMPRSS4 Overexpression Lysate [NBP2-04784]
Western Blot: TMPRSS4 Overexpression Lysate [NBP2-04784] - (Native) [NBP2-04784] - TMPRSS4 detection with empty vector and overexpression lysate. Image from verified customer review. The observed molecular weight is represented by the band at approximately 48 kDa.![Western Blot: TMPRSS4 Overexpression Lysate [NBP2-04784]](https://resources.bio-techne.com/images/products/TMPRSS4-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04784-img0001.jpg "Western Blot: TMPRSS4 Overexpression Lysate [NBP2-04784]")
Western Blot: TMPRSS4 Overexpression Lysate [NBP2-04784]
Western Blot: TMPRSS4 Overexpression Lysate [NBP2-04784] - (Adult Normal) [NBP2-04784] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TMPRSS4. The observed molecular weight is approximately 40 kDa.Formulation, Preparation, and Storage
Formulation
RIPA buffer
Concentration
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at -80C. Avoid freeze-thaw cycles.
Background: TMPRSS4
TMPRSS4 has also been shown to play a role in the process of viral entry for coronaviruses and influenza viruses thru proteolytic activation of key viral glycoproteins. One pathway for SARS-CoV-2 infection, which causes the COVID-19 disease, involves binding of the SARS-CoV-2 Spike 'S' glycoprotein to the human ACE2 receptor and cleavage of S by TMPRSS2 or potentially by TMPRSS4 at the cell surface to facilitate viral entry (3).
References
1. L de Aberasturi A, Calvo A. (2015) TMPRSS4: An Emerging Potential Therapeutic Target in Cancer. Br J Cancer. 112(1):4-8. PMID: 25203520
2. Zeng P., Zhang P., Zhou L., Tang M., Shen Y., Jin J., Zhu Y., Chen M. (2016) TMPRSS4 as an emerging potential poor prognostic factor for solid tumors: A systematic review and meta-analysis. Oncotarget. 7:76327-76336. PMID: 27344186
3. Zang F, Castro MFG, McCune BT, Zeng Q, Rothlauf PW, Sonnek NM, Liu Z, Brulois KF, Wang X, Greenberg HB, Diamond MS, Ciorba MA, Whelan SPJ, and Ding S. (2020) TMPRSS2 and TMPRSS4 promote SARS-CoV-2 infection of human small intestinal enterocytes. Sci Immunol. 5(47): eabc3582. PMID: 32404436
Long Name
Transmembrane protease, serine 4
Alternate Names
CAPH2, MT-SP2
Gene Symbol
TMPRSS4
Additional TMPRSS4 Products
Product Documents for TMPRSS4 Overexpression Lysate
Product Specific Notices for TMPRSS4 Overexpression Lysate
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.
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