v6.5 Mouse embryonic stem cells Whole Cell Lysate

Mouse

Immunoprecipitation, Western Blot

This lysate is prepared as a positive control for immunoprecipitation or separation by SDS-PAGE and subsequent Western blot analysis. Lysates are prepared in denaturing buffer WITHOUT loading buffer or reducing agents (i.e. 2-mercaptoethanol (BME) or dithiothreitol (DTT)). If reducing conditions are desired, add a reducing agent prior to heating. Heat lysate to 95 degrees C for 5 minutes and rapidly cool. The recommended loading amount is 0.01-0.03 mg per lane.

Cell

Embryo

Whole

Standard RIPA buffer including protease and phosphatase inhibitors and 0.1% SDS. No reducing agents or loading buffer added.

No Preservative

3.0 mg/ml

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Store at -80C. Avoid freeze-thaw cycles.