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Recombinant Mouse AHSA1 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18324

Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-18324-100ug
NBP3-18324-200ug
NBP3-18324-50ug

Key Product Details

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE, Western Blot

Product Specifications

Description

A recombinant protein corresponding to mouse AHSA1.

Source: E. coli

Amino Acid Sequence: MGHHHHHHMAKWGEGDPRWIVEERADATNVNNWHWTERDASNWSTEKLKTLFLAVRVENEEGKCEVTEVNKLDGEASINNRKGKLIFFYEWTIKLNWTGTSKSGVQYKGHVEIPNLSDENSVDEVEISVSLAKDEPDTNLVALMKEDGVKLLREAVGIYISTLKTEFTQGMILPTVNGESVDPVGQPALKTETCKAKSAPSKSQAKPVGVKIPTCKITLKETFLTSPEELYRVFTTQELVQAFTHAPAALEADRGGKFHMVDGNVTGEFTDLVPEKHIAMKWRFKSWPEGHFATITLTFIDKNGETELCMEGRGIPAPEEERTRQGWQRYYFEGIKQTFGYGARLF

Purity

>85%

Predicted Molecular Mass

38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

2uM generated a 9-fold ATPase activation of 2uM HSP90 (His-tagged HSP90 beta) in 33mM Hepes pH7.2, 30mM NaCl, 5mM MgCl2, 1mM DTT, 1.5mM ATP in a 100ul reaction at 37 degrees C. (This is an enzyme-linked ATP regeneration assay tracking loss of NADH absorbance at 340nm.)

Localization

Cytoplasm

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse AHSA1 His Protein

SDS-PAGE: Recombinant Mouse AHSA1 His Protein [NBP3-18324]

SDS-PAGE: Recombinant Mouse AHSA1 His Protein [NBP3-18324]

SDS-Page: Recombinant Mouse AHSA1 His Protein [NBP3-18324] - SDS-PAGE of ~38kDa his-tagged mouse AHSA1 protein (NBP3-18324).

Formulation, Preparation and Storage

NBP3-18324
Preparation Method This protein is affinity purified.
Formulation 25mM Hepes buffer pH7.2, 100mM KoAc, 50% glycerol
Preservative No Preservative
Concentration Please see the vial label for concentration. If unlisted please contact technical services.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20C. Avoid freeze-thaw cycles.

Background: AHSA1

Cochaperone that stimulates HSP90 ATPase activity . May affect a step in the endoplasmic reticulum to Golgi trafficking

Alternate Names

activator of 90 kDa heat shock protein ATPase homolog 1, AHA1, activator of heat shock 90kDa protein ATPase homolog 1 (yeast), C14orf3, chromosome 14 open reading frame 3, p38AHA1

Gene Symbol

AHSA1

Additional AHSA1 Products

Product Documents for Recombinant Mouse AHSA1 His Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Mouse AHSA1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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