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Recombinant Human AICDA His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22671

Novus Biologicals, part of Bio-Techne
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NBP2-22671

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-198 of Human AICDA

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MDSLLMNRRK FLYQFKNVRW AKGRRETYLC YVVKRRDSAT SFSLDFGYLR NKNGCHVELL FLRYISDWDL DPGRCYRVTW FTSWSPCYDC ARHVADFLRG NPNLSLRIFT ARLYFCEDRK AEPEGLRRLH RAGVQIAIMT FKDYFYCWNT FVENHERTFK AWEGLHENSV RLSRQLRRIL LPLYEVDDLR DAFRTLGL

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

26.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human AICDA His Protein

SDS-PAGE: Recombinant Human AICDA His Protein [NBP2-22671]

SDS-PAGE: Recombinant Human AICDA His Protein [NBP2-22671]

SDS-Page: Recombinant Human AICDA Protein [NBP2-22671]

Formulation, Preparation and Storage

NBP2-22671
Formulation 20 mM Tris-HCl buffer (pH 8.0), 0.4M UREA, 10% glycerol
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AICDA

AICDA, also known as AID, is involved in somatic hypermutation, gene conversion, and class-switch recombination in B-lymphocytes. This protein is required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses. It may also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation. Recombinant human AICDA protein, fused to His-tag at N-terminus, was expressed in E.coli.

Long Name

Activation-Induced Cytidine Deaminase

Alternate Names

AID, Arp2, CDA2, HIGM2

Gene Symbol

AICDA

Additional AICDA Products

Product Documents for Recombinant Human AICDA His Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human AICDA His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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