Recombinant Human AKR1D1 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51026

Novus Biologicals, part of Bio-Techne
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NBP1-51026-0.1mg
NBP1-51026-0.5mg
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all AKR1D1 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-326 of Human AKR1D1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MDLSAASHRI PLSDGNSIPI IGLGTYSEPK STPKGACATS VKVAIDTGYR HIDGAYIYQN EHEVGEAIRE KIAEGKVRRE DIFYCGKLWA TNHVPEMVRP TLERTLRVLQ LDYVDLYIIE VPMAFKPGDE IYPRDENGKW LYHKSNLCAT WEAMEACKDA GLVKSLGVSN FNRRQLELIL NKPGLKHKPV SNQVECHPYF TQPKLLKFCQ QHDIVITAYS PLGTSRNPIW VNVSSPPLLK DALLNSLGKR YNKTAAQIVL RFNIQRGVVV IPKSFNLERI KENFQIFDFS LTEEEMKDIE ALNKNVRFVE LLMWRDHPEY PFHDEY

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

39.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human AKR1D1 His Protein

SDS-PAGE: Recombinant Human AKR1D1 His Protein [NBP1-51026]

SDS-PAGE: Recombinant Human AKR1D1 His Protein [NBP1-51026]

SDS-Page: Recombinant Human AKR1D1 His Protein [NBP1-51026] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation and Storage

NBP1-51026
Formulation 20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 20% glycerol, 100 mM NaCl
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AKR1D1

Aldo-keto reductase family 1, member D1, also known as AKR1D1, is a member of the AKR superfamily. The AKR family of proteins are soluble NADPH oxidoreductases. They play important roles in the metabolism of drugs, carcinogens and reactive aldehydes. AKR1D1 is responsible for the catalysis of the 5-beta-reduction of bile acid intermediates and steroid hormones which carry a delta (4)-3-one structure. AKR1D1 is highly expressed in liver, colon and testis. Deficiency of this enzyme may contribute to hepatic dysfunction. Recombinant human AKR1D1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

3o5bred, Aldo-keto reductase family 1 member D1,3-oxo-5-beta-steroid 4-dehydrogenase, aldo-keto reductase family 1, member D1 (delta4-3-ketosteroid-5-beta-reductase), CBAS2, Delta(4)-3-oxosteroid 5-beta-reductase, EC 1.3.1.3, SRD5B1beta polypeptide 1 (3-oxo-5 beta-steroid delta4-dehydrogenase beta 1)

Gene Symbol

AKR1D1

Additional AKR1D1 Products

Product Documents for Recombinant Human AKR1D1 His Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human AKR1D1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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