Recombinant Human AKR7A3 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-40394

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP1-40394
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

Enzyme Activity, SDS-PAGE

View all AKR7A3 Proteins and Enzymes »

Product Specifications

Description

A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-331 of Human AKR7A3

Source: E.coli

Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSELEM SRQLSRARPA TVLGAMEMGR RMDAPTSAAV TRAFLERGHT EIDTAFVYSE GQSETILGGL GLRLGGSDCR VKIDTKAIPL FGNSLKPDSL RFQLETSLKR LQCPRVDLFY LHMPDHSTPV EETLRACHQL HQEGKFVELG LSNYAAWEVA EICTLCKSNG WILPTVYQGM YNAITRQVET ELFPCLRHFG LRFYAFNPLA GGLLTGKYKY EDKDGKQPVG RFFGNTWAEM YRNRYWKEHH FEGIALVEKA LQAAYGASAP SMTSATLRWM YHHSQLQGAH GDAVILGMSS LEQLEQNLAA AEEGPLEPAV VDAFNQAWHL VAHECPNYFR

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

41.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Specific activity is > 700 pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction 1.0 pmole of 1,2-Naphthoquinone presence of NADPH per minute at pH 7.0 at 25C.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human AKR7A3 His Protein

SDS-PAGE: Recombinant Human AKR7A3 His Protein [NBP1-40394]

SDS-PAGE: Recombinant Human AKR7A3 His Protein [NBP1-40394]

SDS-Page: Recombinant Human AKR7A3 Protein [NBP1-40394] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation and Storage

NBP1-40394
Formulation PBS (pH 7.4), 10% glycerol
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AKR7A3

Aldo-keto reductase family 7, member A 3, also known as AKR7A3, is a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member includes a number of related monomeric NADPH-dependent oxidoreductases, such as aldehyde reductase, aldose reductase, prostaglandin F synthase, xylose reductase, rho crystallin, and many others. AKR7A3 is involved in the detoxification of aldehydes and ketones. The activity of AKR7A3 may detoxify the aflatoxin B1 (AFB1) dialdehyde, which reacts with proteins, and thereby inhibits AFB 1 induced toxicity. Recombinant human AKR7A3, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

AFAR2, AFB1 aldehyde reductase 2, AFB1-AR 2, aflatoxin B1 aldehyde reductase 2, aflatoxin B1 aldehyde reductase member 3, aldo-keto reductase family 7, member A3 (aflatoxin aldehyde reductase)

Gene Symbol

AKR7A3

Additional AKR7A3 Products

Product Documents for Recombinant Human AKR7A3 His Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human AKR7A3 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.