Recombinant Human alcohol dehydrogenase 1A His Protein

Name: Recombinant Human alcohol dehydrogenase 1A His Protein
Brand: Novus Biologicals
SKU: NBP1-99087

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-99087

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Product Specifications
Scientific Data
Preparation & Storage
Background
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Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all alcohol dehydrogenase 1A Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-375 of Human alcohol dehydrogenase 1A

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSTAGKVIKC KAAVLWELKK PFSIEEVEVA PPKAHEVRIK MVAVGICGTD DHVVSGTMVT PLPVILGHEA AGIVESVGEG VTTVKPGDKV IPLAIPQCGK CRICKNPESN YCLKNDVSNP QGTLQDGTSR FTCRRKPIHH FLGISTFSQY TVVDENAVAK IDAASPLEKV CLIGCGFSTG YGSAVNVAKV TPGSTCAVFG LGGVGLSAIM GCKAAGAARI IAVDINKDKF AKAKELGATE CINPQDYKKP IQEVLKEMTD GGVDFSFEVI GRLDTMMASL LCCHEACGTS VIVGVPPDSQ NLSMNPMLLL TGRTWKGAIL GGFKSKECVP KLVADFMAKK FSLDALITHV LPFEKINEGF DLLHSGKSIR TILMF

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human alcohol dehydrogenase 1A His Protein

SDS-PAGE: Recombinant Human alcohol dehydrogenase 1A His Protein [NBP1-99087]

SDS-Page: Recombinant Human alcohol dehydrogenase 1A Protein [NBP1-99087] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation and Storage

NBP1-99087

Formulation	20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 10% glycerol, 0.1 M NaCl
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: alcohol dehydrogenase 1A

Alcohol dehydrogenase 1A, also known as ADH1A, belongs to the alcohol dehydrogenase family. ADH1 is a monomorphic and predominant in fetal and infant livers, becoming less active in gestation and only weakly active during adulthood. ADH1A plays a major role in ethanol metabolism. With the coenzyme NAD, ADH catalyzes the reversible conversion of organic alcohols to ketones or aldehydes. The physiologic function for ADH1A in the liver is the removal of ethanol formed by microorganisms in the intestinal tract. Recombinant human ADH1A protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

ADH, alpha subunit, ADH1alcohol dehydrogenase 1A, alcohol dehydrogenase 1 (class I), alpha polypeptide, alcohol dehydrogenase 1A (class I), alpha polypeptide, Alcohol dehydrogenase subunit alpha, aldehyde reductase, EC 1.1.1, EC 1.1.1.1

Gene Symbol

ADH1A

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COA

Product Specific Notices for Recombinant Human alcohol dehydrogenase 1A His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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